Strain Data Sheet
RBRC06289
Strain Information | |
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| Image | |
| BRC No. | RBRC06289 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6N-Tyms<tm1a(EUCOMM)Hmgu>/D03 |
| Former Common name | |
| H-2 Haplotype | |
| ES Cell line | JM8.N4 [C57BL/6N] |
| Background strain | C57BL/6NTac |
| Appearance | |
| Strain development | |
| Strain description | |
| Colony maintenance | |
| References | |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Tymsthymidylate synthase5Tyms<tm1a(EUCOMM)Hmgu>targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)5En2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site5Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site5Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)5 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)5 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)5hBactP (human beta actin promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyadenylation site5 |
Ordering Information | |
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| Donor DNA | En2 splice acceptor (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, SV40 polyadenylation site, Bacteriophage P1 loxP site, yeast Frt sites, human beta actin promoter, E. coli Neomycin resistance gene |
| Research application | |
| Specific Term and Conditions | The RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively. |
| Depositor | RIKEN BRC (RIKEN BRC) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |