Strain Data Sheet

RBRC06239

Strain Information

Image
BRC No.RBRC06239
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Siglec1<tm1(cre)Mtka>
Former Common nameCD169-Cre, B6-Siglec1/Cre KI
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain development東京薬科大学生命科学部・田中正人先生。B6JN/1 ES細胞を用いて作出。
Strain descriptionCD169 (Siglec1) 遺伝子プロモーター制御下にimproved Cre遺伝子を挿入したマウス。CD169発現細胞特異的にCreリコンビナーゼを産生する。
Colony maintenanceHomozygote x Homozygote [or crossing to C57BL/6JJcl]
References
Codon-improved Cre recombinase (iCre) expression in the mouse.
D R Shimshek, J Kim, M R Hübner, D J Spergel, F Buchholz, E Casanova, A F Stewart, P H Seeburg, R Sprengel
Genesis, 32(1):19-26 (2002). 11835670

Intestinal CD169(+) macrophages initiate mucosal inflammation by secreting CCL8 that recruits inflammatory monocytes.
Kenichi Asano, Naomichi Takahashi, Mikiko Ushiki, Misa Monya, Fumiaki Aihara, Erika Kuboki, Shigetaka Moriyama, Mayumi Iida, Hiroshi Kitamura, Chun-Hong Qiu, Takashi Watanabe, Masato Tanaka
Nat. Commun., 6:7802 (2015). 26193821

Vascular-resident CD169-positive monocytes and macrophages control neutrophil accumulation in the kidney with ischemia-reperfusion injury.
Kazunori Karasawa, Kenichi Asano, Shigetaka Moriyama, Mikiko Ushiki, Misa Monya, Mayumi Iida, Erika Kuboki, Hideo Yagita, Keiko Uchida, Kosaku Nitta, Masato Tanaka
J. Am. Soc. Nephrol., 26, 896-906 (2015). 25266072

Health Report

Examination Date / Room / Rack2026-07-27Room:4-BRack:I囮検査
2026-05-11Room:4-BRack:I囮検査
2026-03-16Room:3-2Rack:C囮検査
2026-01-13Room:3-2Rack:C囮検査
2025-11-04Room:3-2Rack:C囮検査
2025-08-18Room:3-2Rack:C囮検査
2025-06-09Room:3-2Rack:C囮検査
2025-03-10Room:3-2Rack:C囮検査
2024-12-09Room:3-2Rack:C囮検査

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Siglec1
MGI:99668		sialic acid binding Ig-like lectin 1, sialoadhesin2Siglec1<tm1(cre)Mtka>
MGI:1934021		targeted mutation 1, Masato Tanaka
iCre phage P1 codon improved Cre (iCre)2iCre
neo neomycin resistance gene (E. coli)2 mouse phosphoglycerate kinase promoter (PGK promoter)
polyoma virus polyA2

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • normal phenotype (MP:0002873)
    • Detailed phenotype data

    Ordering Information

    供与核酸Phage P1 improved Cre recombinase, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, polyoma virus polyA, mouse Siglec1 genomic DNA
    Research applicationCre/loxP system
    Immunology and Inflammation Research
    Cre driver
    提供条件利用者は事前に寄託者(開発者)の提供承諾書を得る。研究成果の公表にあたって寄託者の指定する文献を引用する。J. Am. Soc. Nephrol., 26, 896-906 (2015). 研究成果の公表にあたって謝辞の表明を必要とする。本件リソースは寄託者/開発者を共著とする共同研究に限る。
    Depositor田中　正人（東京薬科大学）
    Strain Status生体のアイコン生体
    凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    生体マウスを1~3ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Grootveld AK, Kyaw W, Panova V, Lau AWY, Ashwin E, Seuzaret G, Dhenni R, Bhattacharyya ND, Khoo WH, Biro M, Mitra T, Meyer-Hermann M, Bertolino P, Tanaka M, Hume DA, Croucher PI, Brink R, Nguyen A, Bannard O, Phan TG.
    Apoptotic cell fragments locally activate tingible body macrophages in the germinal center.
    Cell 186(6) 1144-1161.e18(2023) 36868219
    Gorki AD, Symmank D, Zahalka S, Lakovits K, Hladik A, Langer B, Maurer B, Sexl V, Kain R, Knapp S.
    Murine Ex Vivo Cultured Alveolar Macrophages Provide a Novel Tool to Study Tissue-Resident Macrophage Behavior and Function.
    Am J Respir Cell Mol Biol 66(1) 64-75(2022) 34586974
    Camara A, Lavanant AC, Abe J, Desforges HL, Alexandre YO, Girardi E, Igamberdieva Z, Asano K, Tanaka M, Hehlgans T, Pfeffer K, Pfeffer S, Mueller SN, Stein JV, Mueller CG.
    CD169+ macrophages in lymph node and spleen critically depend on dual RANK and LTbetaR signaling.
    Proc Natl Acad Sci U S A 119(3) (2022) 35031565
    Millard SM, Heng O, Opperman KS, Sehgal A, Irvine KM, Kaur S, Sandrock CJ, Wu AC, Magor GW, Batoon L, Perkins AC, Noll JE, Zannettino ACW, Sester DP, Levesque JP, Hume DA, Raggatt LJ, Summers KM, Pettit AR.
    Fragmentation of tissue-resident macrophages during isolation confounds analysis of single-cell preparations from mouse hematopoietic tissues.
    Cell Rep 37(8) 110058(2021) 34818538
    Uderhardt S, Martins AJ, Tsang JS, Lämmermann T, Germain RN.
    Resident Macrophages Cloak Tissue Microlesions to Prevent Neutrophil-Driven Inflammatory Damage.
    Cell 177(3) 541-555.e17(2019) 30955887
    Camara A, Cordeiro OG, Alloush F, Sponsel J, Chypre M, Onder L, Asano K, Tanaka M, Yagita H, Ludewig B, Flacher V, Mueller CG.
    Lymph Node Mesenchymal and Endothelial Stromal Cells Cooperate via the RANK-RANKL Cytokine Axis to Shape the Sinusoidal Macrophage Niche.
    Immunity 50(6) 1467-1481.e6(2019) 31201093