Strain Data Sheet

RBRC06236

Strain Information

Image
BRC No.RBRC06236
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Slc30a2<tm1Thir>
Former Common nameZnT2 KO, B6-Slc30a2 KO
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Keigo Nishida, RIKEN Center for Allergy and Immunology. M1 ES cells derived from (C57BL/6 x 129)F1 were used for the generation of knockout mice. C57BL/6 and 129 mixed background.
Strain descriptionZnT2 (Slc30a2) gene knockout mice. Exons 6 through 8 were replaced with a neo cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Essential Role for Zinc Transporter 2 (ZnT2)-mediated Zinc Transport in Mammary Gland Development and Function during Lactation.
Lee S, Hennigar S R, Alam S, Nishida K, Kelleher S L
J. Biol. Chem., 290(21)13064-78 (2015). 25851903

Mast cells play role in wound healing through the ZnT2/GPR39/IL-6 axis.
Nishida K, Hasegawa A, Yamasaki S, Uchida R, Ohashi W, Kurashima Y, Kunisawa J, Kimura S, Iwanaga T, Watarai H, Hase K, Ogura H, Nakayama M, Kashiwakura J I, Okayama Y, Kubo M, Ohara O, Kiyono H, Koseki H, Murakami M, Hirano T
Sci. Rep., 9(1):10842 (2019). 31346193

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Slc30a2
MGI:106637		solute carrier family 30 (zinc transporter), member 24Slc30a2<tm1Thir> targeted mutation 1, Toshio Hirano
  • zinc deficiency, transient neonatal(MedGEN)
    • neo neomycin resistance gene (E. coli)4

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal branching of the mammary ductal tree(MP:0000662)

  • abnormal macrophage physiology(MP:0002451)

  • abnormal mammary gland development(MP:0000628)

  • abnormal mammary gland duct morphology(MP:0009503)

  • abnormal mammary gland epithelium morphology(MP:0009504)
    • more 8 phenotypes
  • abnormal mammary gland growth during lactation(MP:0006270)

  • abnormal mammary gland luminal epithelium morphology(MP:0013781)

  • abnormal zinc homeostasis(MP:0009615)

  • decreased mammary gland epithelial cell proliferation(MP:0010174)

  • hypolactation(MP:0013716)

  • increased mammary gland apoptosis(MP:0014126)

  • increased mammary gland epithelial cell proliferation(MP:0010173)

  • maternal effect(MP:0003718)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Slc30a2 genomic DNA, E. coli Neomycin resistance gene
    Research applicationCell Biology Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 290(21)13064-78 (2015). Sci. Rep., 9(1):10842 (2019).
    DepositorKeigo Nishida (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data