Strain Data Sheet

RBRC06206

Strain Information

Image
BRC No.RBRC06206
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6C3-Tg(Cerl2-sGFP)52Hmd
Former Common nameCerl2(Cerl2-sGFP)BAC(52)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Tetsuya Nakamura and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2008. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. C57BL/6 and C3H mixed background.
Strain descriptionBAC Cerl2-sGFP transgenic mice. The mice were generated by using a BAC clone containing the mouse Cerl2 gene, PR23-421H21. The sGFP (a 300-bp sequence coding for a portion of EGFP) gene was inserted into the exon 2 of in the Cerl2 BAC clone.
Colony maintenanceCarrier x Noncarrier [or Crossing to Jcl:B6C3F1]
References
Fluid flow and interlinked feedback loops establish left-right asymmetric decay of Cerl2 mRNA.
Nakamura T, Saito D, Kawasumi A, Shinohara K, Asai Y, Takaoka K, Dong F, Takamatsu A, Belo J A, Mochizuki A, Hamada H
Nat. Commun., 3:1322 (2012). 23271656

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP mouse Dand5 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Cerberus like 2 gene, Aequorea victoria a portion of EGFP gene
    Research applicationDevelopmental Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 3:1322 (2012). doi: 10.1038/ncomms2319.
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data