Strain Data Sheet

RBRC06199

Strain Information

Image
BRC No.RBRC06199
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(NDE-hsp-GCaMP2,lacZ)1Hmd
Former Common nameNDE-GCaMP2-IZ(1), B6-NDE-GCaMP2/lacZ Tg#1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development大阪大学大学院生命機能研究科・吉場聡子先生、濱田博司合先生ら（2010）。B6C3F1受精卵へのインジェクションにより作出。C57BL/6、C3Hおよび129混合背景。
Strain descriptionノード特異的エンハンサー (NDE) によってノードの両側で、カルシウムセンサーGCaMP2とlacZを発現するマウス。
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
ReferencesScience, 338, 226-231 (2012). 22983710
Nat. Biotechnol., 19, 137-141 (2001). 11175727

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GCaMP2RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP2mouse node-notochord enhancer, mouse Hsp68 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signalUN

Ordering Information

供与核酸mouse node-notochord enhancer, mouse Hsp68 promoter, GCaMP2(RSET tag, chicken myosin light chain kinase (M13), Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, SV40 polyA signal
Research applicationDevelopmental Biology Research
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたって譲渡者の指定する文献を引用する。Science, 338, 226-231 (2012).
Depositor濱田　博司（国立研究開発法人理化学研究所）
Strain Status凍結胚のアイコン凍結胚
Strain Availability受注後、検査を施して提供
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data