Strain Data Sheet

RBRC06156

Strain Information

Image
BRC No.RBRC06156
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Slc5a4a<tm1Csk>
Former Common nameSGLT3a knockout mouse, SGLT3a KO, B6-Slc5a4a KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain development中外製薬株式会社にて開発。C57BL/6Nマウス由来ES細胞を用いて遺伝子ジーンタゲティング法により樹立。C57BL/6N背景。
Strain descriptionSlc5a4a (SGLT3a) 遺伝子のノックアウトマウス。エクソン1から3の一部までをPGK-neoカセットで置換。生存性、繁殖性あり。外見上の異常は認められない。
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Slc5a4asolute carrier family 5, member 4a10Slc5a4a<tm1Csk>targeted mutation 1, Chugai Pharmaceutical Co., Ltd.

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)10mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter

Ordering Information

供与核酸mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter (synthetic), E. coli Neomycine resistance gene, (synthetic) polyA signal, mouse Slc5a4a genomic DNA
Research application
提供条件条件を付加する。
利用者は寄託者と事前に検体提供覚書を締結すること。（連絡先：創薬薬理研究部 福澤拓 <fukuzawatk@chugai-pharm.co.jp>)
Depositor寺社下　浩一（中外製薬株式会社）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data