Strain Data Sheet

RBRC06140

Strain Information

Image
BRC No.RBRC06140
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Fabp4-Serpina12)10
Former Common nameVaspin Tg#10, B6-Serpina12 Tg#10
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain development岡山大学大学院医歯薬学総合研究科・和田　淳先生、東京大学医科学研究所・岩倉洋一郎先生（2005）。C57BL/6J背景。
Strain descriptionVaspin (Serpina12) 遺伝子のトランスジェニックマウス。マウスaP2プロモーター制御下でVaspinを発現する。Vaspinは脂肪組織で産生される分泌蛋白でセリンプロテアーゼインヒビター (SERPIN) ファミリーに属し、α1アンチトリプシンと約40%のホモロジーを有する。ヒト血中では0.1〜数10ng/mlで存在している。肥満や2型糖尿病においてVaspinの発現は代償的に亢進する。Vaspinを脂肪組織に過剰に発現させたマウスでは、高脂肪高ショ糖食飼育時の体重増加やインスリン抵抗性、脂肪肝が改善する。また、血管内皮細胞のアポトーシス抑制を介して動脈硬化が軽減する。肥満やインスリン抵抗性および動脈硬化に関する治療ターゲットの探索などの研究に有用である。
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Vaspin is an adipokine ameliorating ER stress in obesity as a ligand for cell-surface GRP78/MTJ-1 complex.
Atsuko Nakatsuka, Jun Wada, Izumi Iseda, Sanae Teshigawara, Kanji Higashio, Kazutoshi Murakami, Motoko Kanzaki, Kentaro Inoue, Takahiro Terami, Akihiro Katayama, Kazuyuki Hida, Jun Eguchi, Chikage Sato Horiguchi, Daisuke Ogawa, Yasushi Matsuki, Ryuji Hiramatsu, Hideo Yagita, Shigeru Kakuta, Yoichiro Iwakura, Hirofumi Makino
Diabetes, 61, 2823-2832, (2012). 22837305

Visceral adipose tissue-derived serine proteinase inhibitor inhibits apoptosis of endothelial cells as a ligand for the cell-surface GRP78/voltage-dependent anion channel complex.
Atsuko Nakatsuka, Jun Wada, Izumi Iseda, Sanae Teshigawara, Kanji Higashio, Kazutoshi Murakami, Motoko Kanzaki, Kentaro Inoue, Takahiro Terami, Akihiro Katayama, Kazuyuki Hida, Jun Eguchi, Daisuke Ogawa, Yasushi Matsuki, Ryuji Hiramatsu, Hideo Yagita, Shigeru Kakuta, Yoichiro Iwakura, Hirofumi Makino
Circ Res., 112, 771-780 (2013). 23307819

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GH Growth hormone polyA (human)UNGH
HBB2 rabbit beta-globin intronUNHBB2
Serpina12 serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12UNSerpina12 mouse fatty acid binding protein 4, adipocyte promoter

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • decreased body weight (MP:0001262)

  • decreased circulating LDL cholesterol level (MP:0000183)

  • decreased circulating insulin level (MP:0002727)

  • decreased circulating leptin level (MP:0005668)

  • decreased epididymal fat pad weight (MP:0009289)
    • more 4 phenotypes
  • decreased liver triglyceride level (MP:0009356)

  • decreased liver weight (MP:0003402)

  • decreased susceptibility to hepatic steatosis (MP:0002310)

  • increased insulin sensitivity (MP:0002891)
    • Detailed phenotype data

    Ordering Information

    供与核酸Mouse aP2 promoter, Mouse SERPINA12 (vaspin) gene cDNA, Rabbit beta-Globin intron, Human GH polyA signal
    Research applicationApoptosis Research
    Diabetes and Obesity Research
    Hematological Research
    Metabolism Research
    Mouse Models for Human Disease
    covid19_progression
    提供条件条件を付加しない。
    Depositor和田　淳（国立大学法人岡山大学）
    Strain Status凍結精子のアイコン凍結精子
    Strain Availability受注後、検査を施して提供
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data