Strain Data Sheet

RBRC06064

Strain Information

Image
BRC No.RBRC06064
TypeGene Trap CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pknox1<Gt(Ayu21-B132*SACre)2Card>
Former Common namePknox1-SACre,402, B6-Pknox1/Cre Gt#2
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Masatake Araki, Institute of Molecular Embryology and Genetics, Kumamoto University. KTPU8 ES cells were used. C57BL/6 and CBA mixed.
Strain descriptionPknox1-cre driver mice. The beta-geo gene in the exchangeable gene trap clone of Pknox1 gene was replaced with a cre recombinase gene.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)17En2
Frt yeast FRT (flippase recombination target) site17Frt
Pknox1
MGI:1201409		Pbx/knotted 1 homeobox17Pknox1<Gt(Ayu21-B132*SACre)2Card> gene trap 2, Center for Animal Resources & Development
cre Phage P1 Cre recombinase17cre
loxP phage P1 loxP17loxP
loxP phage P1 loxP17loxP
nls SV40 large T antigen nuclear localization signal (NLS)17nls
polyA mouse PGK17polyA
puro puromycin acyltransferase (Streptomyces alboniger)17puro

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse En2 splice acceptor, mouse PGK promoter, mouse PGK polyA signal, Streptomyces alboniger puromycin resistance gene, phage P1 cre recombinase, SV40 nuclear localization signal, yeast FRT (flipase recombination target) site, phage P1 loxP and mutant lox sequence, E. coli pSP73 vector sequence, mouse Pknox1 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. will be announced.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMasatake Araki (Kumamoto University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data