Strain Data Sheet

RBRC06060

Strain Information

Image
BRC No.RBRC06060
TypeGene Trap CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Alad<Gt(Ayu21-T323*mERT2)1Card>
Former Common nameAlad-mERT2,1094, B6-Alad/CreER Gt#1
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Masatake Araki, Institute of Molecular Embryology and Genetics, Kumamoto University. KTPU8 ES cells were used. C57BL/6 and CBA mixed.
Strain descriptionAlad-creER driver mice. The beta-geo gene in the exchangeable gene trap clone of Alad gene was replaced with a mERT2.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Alad
MGI:96853		aminolevulinate, delta-, dehydratase4Alad<Gt(Ayu21-T323*mERT2)1Card> gene trap 1, Center for Animal Resources & Development
  • porphyria due to ALA dehydratase deficiency(MedGEN)
    • En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)4En2
    Ert2 estrogen receptor (mouse)4Ert2
    Frt yeast FRT (flippase recombination target) site4Frt
    cre Phage P1 Cre recombinase4cre
    loxP phage P1 loxP4loxP
    loxP phage P1 loxP4loxP
    nls SV40 large T antigen nuclear localization signal (NLS)4nls
    polyA mouse PGK4polyA
    puro puromycin acyltransferase (Streptomyces alboniger)4puro

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse En2 splice acceptor, mouse PGK promoter, mouse PGK polyA signal, Streptomyces alboniger puromycin resistance gene, phage P1 cre recombinase, mouse estrogen receptor, yeast FRT (flipase recombination target) site, phage P1 loxP and mutant lox sequence, E. coli pSP73 vector sequence, mouse Alad genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. will be announced. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorMasatake Araki (Kumamoto University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data