Strain Data Sheet

RBRC06047

Strain Information

Image
BRC No.RBRC06047
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hes1<tm1Imayo>
Former Common nameB6-Hes1-floxed ; B6-Hes1 floxed
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2007. TT2 ES cells were used. The mutant mice were crossed to C57BL/6 for 6 times.
Strain descriptionHes1 gene floxed mice. LoxP sequences were knocked into the first intron and downstream of 3'UTR of Hes1 gene for conditional knock-out study. PGK-Neo sequence was removed.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
References
Hes genes and neurogenin regulate non-neural versus neural fate specification in the dorsal telencephalic midline.
Itaru Imayoshi, Tomomi Shimogori, Toshiyuki Ohtsuka, Ryoichiro Kageyama
Development, 135(15):2531-2541 (2008). 18579678

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Hes1
MGI:104853		hes family bHLH transcription factor 116Hes1<tm1Kag>
MGI:3808031		targeted mutation 1, Ryoichiro Kageyama
loxP phage P1 loxP16loxP
loxP phage P1 loxP16loxP

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • no abnormal phenotype detected (MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage P1 loxP sites, mouse Hes1 genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 135(15):2531-2541 (2008).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
    DepositorDrs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Yamashita E, Hashimoto S, Abe H, Sudo T, Okuzaki D, Okawa T, Ishii M.
    Red Blood Cell-Mediated Enhancement of Hematopoietic Stem Cell Functions via a Hes1-Dependent Pathway.
    FASEB J 39(17) e71022(2025) 40923227
    Fujimaki S, Matsumoto T, Muramatsu M, Nagahisa H, Horii N, Seko D, Masuda S, Wang X, Asakura Y, Takahashi Y, Miyamoto Y, Usuki S, Yasunaga KI, Kamei Y, Nishinakamura R, Minami T, Fukuda T, Asakura A, Ono Y.
    The endothelial Dll4-muscular Notch2 axis regulates skeletal muscle mass.
    Nat Metab 4(2) 180-189(2022) 35228746
    Ma Z, Xu J, Wu L, Wang J, Lin Q, Chowdhury FA, Mazumder MHH, Hu G, Li X, Du W.
    Hes1 deficiency causes hematopoietic stem cell exhaustion.
    Stem Cells 38(6) 756-768(2020) 32129527