Strain Data Sheet

RBRC05998

Strain Information

Image
BRC No.RBRC05998
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Nes-cre/ERT2)4Imayo
Former Common nameNestin-CreERT2 Line4 ; B6-Nes-CreER#4
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2005. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs.
Strain descriptionNestin-CreER transgenic mice. CreERT2 is expressed by neural stem/progenitor cells. CreERT2-expression level is intermediate.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Temporal regulation of Cre recombinase activity in neural stem cells.
Itaru Imayoshi, Toshiyuki Ohtsuka, Daniel Metzger, Pierre Chambon, Ryoichiro Kageyama
Genesis, 44(5):233-8 (2006). 16652364

Health Report

Examination Date / Room / Rack2025/12/22Room:4-ARack:HSentinel mouse program
2025/10/14Room:4-ARack:HSentinel mouse program
2025/07/28Room:4-ARack:HSentinel mouse program
2025/05/19Room:4-ARack:HSentinel mouse program
2025/02/17Room:4-ARack:HSentinel mouse program
2024/11/18Room:4-ARack:HSentinel mouse program
2024/08/19Room:4-ARack:HSentinel mouse program
2024/05/20Room:4-ARack:HSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ERT2 estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)UNERT2
cre Phage P1 Cre recombinaseUNcre
SV40 polyA signalUN

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • no abnormal phenotype detected (MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNARat Nestin promoter, Nestin 2nd intron enhancer, SV40 PolyA signal, Cre recombinase (P1 pharge), human ER ligand binding domain (ERT2)
    Research applicationCre/loxP system
    Neurobiology Research
    Cre driver
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 44(5):233-8 (2006). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
    DepositorDrs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Cre-ERT(2): NOTICE TO RECIPIENT
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Baba Y, Onishi-Sakamoto S, Ide K, Nishifuji K.
    Nestin is a marker of unipotent embryonic and adult progenitors differentiating into an epithelial cell lineage of the hair follicles.
    Sci Rep 12(1) 17820(2022) 36280775