Strain Data Sheet

RBRC05983

Strain Information

Image
BRC No.RBRC05983
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameICR.Cg-Hes7<tm1Kag>
Former Common nameHes7 KO ; Hes7 KO/lacZ
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2001. TT2 ES cells were used. The mutant mice were crossed to ICR.
Strain descriptionMost of the coding region was replaced with a IRES-lacZ-PGK-neo cassette. Most homozygous mutant mice die within a few hours of birth.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Slc:ICR]
References
Dynamic expression and essential functions of Hes7 in somite segmentation.
Bessho Y, Sakata R, Komatsu S, Shiota K, Yamada S, Kageyama R
Genes Dev. 2001 15(20):2642-7. 11641270

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Hes7
MGI:2135679		hes family bHLH transcription factor 711Hes7<tm1Kag>
MGI:2652222		targeted mutation 1, Ryoichiro Kageyama
  • spondylocostal dysostosis 4, autosomal recessive(MedGEN)
    • Ires internal ribosomal entry site (EMCV)11
    lacZ beta-galactosidase (E. coli)11
    neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal dorsal-ventral polarity of the somites(MP:0005223)

  • abnormal intervertebral disk development(MP:0005229)

  • abnormal left-right axis symmetry of the somites(MP:0005224)

  • abnormal rib morphology(MP:0000150)

  • abnormal sacral vertebrae morphology(MP:0003050)
    • more 21 phenotypes
  • abnormal skeleton morphology(MP:0005508)

  • abnormal somite development(MP:0001688)

  • abnormal somite shape(MP:0001691)

  • abnormal somite size(MP:0005222)

  • abnormal vertebrae morphology(MP:0000137)

  • abnormal vertebral arch morphology(MP:0004599)

  • abnormal vertebral body development(MP:0005227)

  • abnormal vertebral body morphology(MP:0000141)

  • abnormal vertebral column morphology(MP:0004703)

  • decreased body length(MP:0001258)

  • decreased rib number(MP:0003345)

  • fused somites(MP:0011733)

  • fusion of vertebral arches(MP:0004613)

  • fusion of vertebral bodies(MP:0004612)

  • incomplete somite formation(MP:0001689)

  • kinked tail(MP:0000585)

  • neonatal lethality, complete penetrance(MP:0011087)

  • rib bifurcation(MP:0000153)

  • rib fusion(MP:0000154)

  • short tail(MP:0000592)

  • vertebral fusion(MP:0004609)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEMCV (Encephalomyocarditis virus) internal ribosomal entry site (IRES), beta galactosidase (E. coli), mouse phosphoglycerate kinase promoter (PGK promoter), Neo (E. coli), mouse Hes7 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev. 2001 15(20):2642-7.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
    DepositorDrs. Itaru Imayoshi and Ryoichiro Kageyama (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data