Strain Data Sheet

RBRC05977

Strain Information

Image
BRC No.RBRC05977
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Mmp14<tm2Ski>
Former Common nameMT1-MMP(MMP14) knockout mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain development東京大学医科学研究所・清木元治先生、勝木元也先生。E14 ES細胞由来。C57BL/6Jに戻し交配された（15回以上）。
Strain descriptionMT1-MMP (Mmp14) 遺伝子のノックアウトマウス (lacZノックインマウス) 。ホモマウスはコラーゲン代謝異常により骨形成異常や繊維症を呈する。生後2-3週間で死亡。lacZノックイン型のため、MT1-MMP遺伝子の生体での発現解析が容易。
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesGenes Cells., 14, 617-626 (2009). 19371380
J. Cell Sci., 120, 1607-1614 (2007). 17405818

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mmp14matrix metallopeptidase 14 (membrane-inserted)14Mmp14<tm2Ski>targeted mutstion 2, Motoharu Seiki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
gptguanine phosphoribosyltransferase14gptmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)14

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)14herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)14nls

Ordering Information

供与核酸SV40 nuclear localization signal (NLS), E. coli LacZ, mouse Phosphoglcerate kinase promoter (PGK promoter), E. coli gpt, HSV Thymidine kinase promoter, E.coli neomycin resistance gene, SV40 PolyA signal
Research applicationDermatology Research
Developmental Biology Research
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Genes Cells., 14, 617-626 (2009).
Depositor清木　元治（国立大学法人東京大学）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data