Strain Data Sheet
RBRC05967
Strain Information | |
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| Image | |
| BRC No. | RBRC05967 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | C57BL/6N-Efcab1<tm1a(KOMP)Osb>/11 |
| Former Common name | Efcab1 KO |
| H-2 Haplotype | |
| ES Cell line | EGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb] |
| Background strain | C57BL/6NJcl |
| Appearance | |
| Strain development | 大阪大学微生物病研究所・伊川正人先生（2012）。 |
| Strain description | 全身で広く発現するEfcab1遺伝子のエクソン4をloxP配列で挟んだfloxedマウス。ライン11。 |
| Colony maintenance | Heterozygote x Wild-type [C57BL/6NJcl] |
| References | Commun. Biol., 2:226 (2019). doi: 10.1038/s42003-019-0462-y. 31240264 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Efcab1EF hand calcium binding domain 116Efcab1<tm1a(KOMP)Osb>targeted mutation 1a, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)16En2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site16Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site16Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)16 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)16 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP16loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP16loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP16loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)16human beta-actin promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyadenylation site16 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyadenylation site16 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)UNCAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV_IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N_terminal peptide Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)UNGH |
Ordering Information | |
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| 供与核酸 | En2 splice acceptor (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, SV40 polyadenylation site, hBactP (human beta-actin promoter), E.coli neomycin phosphotransferase (neoR) gene, yeast FRT (flipase recombination target) sites, phage P1 loxP, mouse Efcab1 genomic DNA, Jellyfish GFP cDNA, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV-IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, bovine growth hormone polyadenylation signal |
| Research application | Cre/loxP system FLP/frt system Reproductive Biology Research |
| 提供条件 | 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。 研究成果の公表にあたって寄託者の指定する文献を引用する。Commun. Biol., 2:226 (2019). doi: 10.1038/s42003-019-0462-y. 使用範囲は学術機関の学術研究に限る。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。本件リソースがKnockout Mouse ProjectおよびKOMP Repositoryを由来としていることを明記すること。 |
| Depositor | 伊川 正人（国立大学法人大阪大学） |
| Strain Status |  凍結精子 |
| Strain Availability |  受注後、検査を施して提供 |
| Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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