Strain Data Sheet

RBRC05967

Strain Information

Image
BRC No.RBRC05967
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Efcab1<tm1a(KOMP)Osb>/11
Former Common nameEfcab1 KO
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strainC57BL/6NJcl
Appearance
Strain developmentDeveloped by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2012.
Strain descriptionEfcab1 gene floxed mice. Exon 4 was flanked by loxP sites.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NJcl]
References
Calaxin is required for cilia-driven determination of vertebrate laterality.
Sasaki K, Shiba K, Nakamura A, Kawano N, Satouh Y, Yamaguchi H, Morikawa M, Shibata D, Yanase R, Jokura K, Nomura M, Miyado M, Takada S, Ueno H, Nonaka S, Baba T, Ikawa M, Kikkawa M, Miyado K, Inaba K
Commun. Biol., 2:226 (2019). 31240264

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Efcab1
MGI:1914043		EF hand calcium binding domain 116Efcab1<tm1a(KOMP)Osb> targeted mutation 1a, Research Institute for Microbial Diseases, Osaka University
En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)16En2
Frt yeast FRT (flippase recombination target) site16Frt
Frt yeast FRT (flippase recombination target) site16Frt
Ires internal ribosomal entry site (EMCV)16
lacZ beta-galactosidase (E. coli)16
loxP phage P1 loxP16loxP
loxP phage P1 loxP16loxP
loxP phage P1 loxP16loxP
neo neomycin resistance gene (E. coli)16 human beta-actin promoter
SV40 polyadenylation site16
SV40 polyadenylation site16
GFP Green Fluorescent Protein (Aequorea victoria)UN CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV_IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N_terminal peptide
GH Growth hormone polyA (Bovine)UNGH

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEn2 splice acceptor (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, SV40 polyadenylation site, hBactP (human beta-actin promoter), E.coli neomycin phosphotransferase (neoR) gene, yeast FRT (flipase recombination target) sites, phage P1 loxP, mouse Efcab1 genomic DNA, Jellyfish GFP cDNA, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV-IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, bovine growth hormone polyadenylation signal
    Research applicationCre/loxP system
    FLP/frt system
    Reproductive Biology Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Commun. Biol., 2:226 (2019). doi: 10.1038/s42003-019-0462-y.Using the BIOLOGICAL RESOURCE is limited to the academic research of academic institutions. The RECIPIENT must contact in advance the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.　The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
    DepositorMasahito Ikawa (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP

    BRC mice in Publications

    No Data