Strain Data Sheet
RBRC05965
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC05965 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | STOCK Fer1l6<tm1a(KOMP)Osb>/91 |
| Former Common name | Fer1l6 KO |
| H-2 Haplotype | |
| ES Cell line | EGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)] |
| Background strain | Jcl:B6D2F1 |
| Appearance | |
| Strain development | Developed by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2012. |
| Strain description | Fer1l6 gene floxed mice. Exons 4 and 5 were flanked by loxP sites. |
| Colony maintenance | Heterozygote x Wild-type [or Crossing to Jcl:B6D2F1] |
| References | Testis-enriched ferlin, FER1L5, is required for Ca2+-activated acrosome reaction and male fertility. Akane Morohoshi, Haruhiko Miyata, Keizo Tokuhiro, Rie Iida-Norita, Taichi Noda, Yoshitaka Fujihara, Masahito Ikawa Sci. Adv., 9(4):eade7607 (2023). 36696506 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| En2 | En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence) | 15 | En2 | ||||
| Fer1l6 MGI:3645398 | fer-1 like family member 6 | 15 | Fer1l6<tm1a(KOMP)Osb> | targeted mutation 1a, Research Institute for Microbial Diseases, Osaka University | |||
| Frt | yeast FRT (flippase recombination target) site | 15 | Frt | ||||
| Frt | yeast FRT (flippase recombination target) site | 15 | Frt | ||||
| Ires | internal ribosomal entry site (EMCV) | 15 | |||||
| lacZ | beta-galactosidase (E. coli) | 15 | |||||
| loxP | phage P1 loxP | 15 | loxP | ||||
| loxP | phage P1 loxP | 15 | loxP | ||||
| loxP | phage P1 loxP | 15 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 15 | human beta-actin promoter | ||||
| SV40 polyadenylation site | 15 | ||||||
| SV40 polyadenylation site | 15 | ||||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | En2 splice acceptor (mouse En2 intron 2/exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E.coli LacZ, SV40 polyadenylation site, hBactP (human beta-actin promoter), E.coli neomycin phosphotransferase (neoR) gene, yeast FRT (flipase recombination target) sites, phage P1 loxP, mouse Fer1l6 genomic DNA【クラス１】 |
| Research application | Cre/loxP system FLP/frt system Reproductive Biology Research |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Adv. Jan 25;9(4):eade7607.(2023) doi: 10.1126/sciadv.ade7607. Epub 2023 Jan 25.Using the BIOLOGICAL RESOURCE is limited to the academic research of academic institutions. The RECIPIENT must contact in advance the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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| No Data |