Strain Information | |
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Image | |
BRC No. | RBRC05964 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6N-Tulp2<tm1Osb>/1B |
Former Common name | Tulp2 KO |
H-2 Haplotype | |
ES Cell line | EGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb] |
Background strain | C57BL/6NJcl |
Appearance | |
Strain development | 大阪大学微生物病研究所・藤原祥高先生、伊川正人先生(2012)。 |
Strain description | 精巣特異的に発現するTulp2遺伝子のノックアウトマウス。エクソン7がFrtで挟まれたneoカセットで置換。ホモマウスは見かけ上正常に発育するが、ホモ雄は不妊。ライン1B。 |
Colony maintenance | Heterozygote x Wild-type [C57BL/6NJcl] |
References | Reprod. Med. Biol., 21(1):e12467 (2022). doi: 10.1002/rmb2.12467. eCollection Jan-Dec 2022. 35619658 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Tulp2tubby-like protein 27Tulp2<tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site7Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site7Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)7 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)UNCAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV_IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N_terminal peptide Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)UNGH |
Ordering Information | |
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供与核酸 | mouse Tulp2 genomic DNA, E.coli Neomycin resistance gene, yeast FRT (flipase recombination target) sites, Phage P1 loxP, Jellyfish GFP cDNA, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV-IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, bovine growth hormone polyadenylation signal |
Research application | Cre/loxP system FLP/frt system Reproductive Biology Research |
提供条件 | 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。 研究成果の公表にあたって寄託者の指定する文献を引用する。Reprod. Med. Biol., 21(1):e12467 (2022). doi: 10.1002/rmb2.12467. eCollection Jan-Dec 2022. 非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること(https://www.ccb.osaka-u.ac.jp/en/)。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。 |
Depositor | 伊川 正人(国立大学法人大阪大学) |
Strain Status | 凍結精子 |
Strain Availability | 受注後、検査を施して提供 |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |