Strain Data Sheet
RBRC05960
Strain Information | |
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| Image | |
| BRC No. | RBRC05960 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | STOCK Tulp2<tm1Osb>/3B |
| Former Common name | Tulp2 KO |
| H-2 Haplotype | |
| ES Cell line | EGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)] |
| Background strain | Jcl:B6D2F1 |
| Appearance | |
| Strain development | Developed by Yoshitaka Fujihara and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2012. |
| Strain description | Tulp gene knockout mice. Homozygous mutant mice are viable, but homozygous mutant males were infertile. |
| Colony maintenance | Heterozygote x Wild-type [or Crossing to Jcl:B6D2F1] |
| References | TULP2 deletion mice exhibit abnormal outer dense fiber structure and male infertility. Yuki Oyama, Haruhiko Miyata, Keisuke Shimada, Tamara Larasati, Yoshitaka Fujihara, Masahito Ikawa Reprod. Med. Biol., 21(1):e12467 (2022). 35619658 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Frt | yeast FRT (flippase recombination target) site | 7 | Frt | ||||
| Frt | yeast FRT (flippase recombination target) site | 7 | Frt | ||||
| Tulp2 MGI:1861600 | TUB like protein 2 | 7 | Tulp2<tm1Osb> | targeted mutation 1, Research Institute for Microbial Diseases, Osaka University | |||
| loxP | phage P1 loxP | 7 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 7 | |||||
| GFP | Green Fluorescent Protein (Aequorea victoria) | UN | CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV_IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N_terminal peptide | ||||
| GH | Growth hormone polyA (Bovine) | UN | GH | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | mouse Tulp2 genomic DNA, E.coli Neomycin resistance gene, yeast FRT (flipase recombination target) sites, Phage P1 loxP, Jellyfish GFP cDNA, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV-IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N-terminal peptide, bovine growth hormone polyadenylation signal |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Reprod. Med. Biol., 21(1):e12467 (2022). doi: 10.1002/rmb2.12467. eCollection Jan-Dec 2022.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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| No Data |