Strain Data Sheet

RBRC05928

Strain Information

Image
BRC No.RBRC05928
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Map3k5<tm1Hijo> Map3k6<tm1Hijo> Map3k15<tm1Hijo>
Former Common nameASK1/2/3-TKO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain development東京大学大学院薬学系研究科・一條秀憲先生。Map3k5ノックアウトマウス、Map3k6ノックアウトマウスおよびMap3k15ノックアウトマウス（ともにC57BL/6Jコンジェニック系統）の掛け合わせにより樹立。
Strain descriptionMap3k5 (ASK1) 、Map3k6 (ASK2) およびMap3k15 (ASK3) 遺伝子のトリプルノックアウトマウス。Map3k5は活性酸素や病原体感染などのストレスに応答して活性化するストレス応答MAP3キナーゼであり、アポトーシスやサイトカイン産生を誘導する。Map3k6は、相同性が高いMAP3K5と複合体を形成して活性化し、協調してアポトーシス誘導などに働く。MAP3K15は浸透圧変化などのストレスに応答して活性化するストレス応答MAP3キナーゼである。トリプルホモノックアウトマウスは正常に産まれてくる。Map3k5アレルのGFPの発現は確認されていない。
Colony maintenanceMap3k5: Homozygote x Homozygote; Map3k6: Homozygote x Homozygote; Map3k15: Homozygote (female) x Hemizygote (male) [or Crossing to C57BL/6JJcl]
References
ASK1 is required for sustained activations of JNK/p38 MAP kinases and apoptosis.
K Tobiume, A Matsuzawa, T Takahashi, H Nishitoh, K Morita, K Takeda, O Minowa, K Miyazono, T Noda, H Ichijo
EMBO rep., 2, 222-228 (2001). 11266364

Apoptosis signal-regulating kinase (ASK) 2 functions as a mitogen-activated protein kinase kinase kinase in a heteromeric complex with ASK1.
Kohsuke Takeda, Rieko Shimozono, Takuya Noguchi, Tsuyoshi Umeda, Yoshifumi Morimoto, Isao Naguro, Kei Tobiume, Masao Saitoh, Atsushi Matsuzawa, Hidenori Ichijo
J. Biol. Chem., 282, 7522-7531 (2007). 17210579

ASK1 is required for sustained activations of JNK/p38 MAP kinases and apoptosis.
K Tobiume, A Matsuzawa, T Takahashi, H Nishitoh, K Morita, K Takeda, O Minowa, K Miyazono, T Noda, H Ichijo
EMBO J., 28, 843-853 (2009). 11266364

ASK3 responds to osmotic stress and regulates blood pressure by suppressing WNK1-SPAK/OSR1 signaling in the kidney.
Isao Naguro, Tsuyoshi Umeda, Yumie Kobayashi, Junichi Maruyama, Kazuki Hattori, Yutaka Shimizu, Keiichiro Kataoka, Shokei Kim-Mitsuyama, Shinichi Uchida, Alain Vandewalle, Takuya Noguchi, Hideki Nishitoh, Atsushi Matsuzawa, Kohsuke Takeda, Hidenori Ichijo
Nat Commun. (2012). 23250415

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)10
GH Growth hormone polyA (Bovine)10GH
Map3k5
MGI:1346876		mitogen-activated protein kinase kinase kinase 510Map3k5<tm1Hijo>
MGI:3046838		targeted mutation 1, Hidenori Ichijo
neo neomycin resistance gene (E. coli)10 mouse phosphoglycerate kinase promoter (PGK promoter)
GH Growth hormone polyA (Bovine)4GH
Map3k6
MGI:1855691		mitogen-activated protein kinase kinase kinase 64Map3k6<tm1Hijo>
MGI:3843985		targeted mutation 1, Hidenori Ichijo
lacZ beta-galactosidase (E. coli)4
neo neomycin resistance gene (E. coli)4 mouse phosphoglycerate kinase promoter (PGK promoter)
nls SV40 large T antigen nuclear localization signal (NLS)4nls
Map3k15
MGI:2448588		mitogen-activated protein kinase kinase kinase 15XMap3k15<tm1Hijo> targeted mutation 1, Hidenori Ichijo
loxP phage P1 loxPXloxP
loxP phage P1 loxPXloxP
neo neomycin resistance gene (E. coli)X

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal cell physiology (MP:0005621)

  • abnormal kidney physiology (MP:0002136)

  • abnormal response to cardiac infarction (MP:0000343)

  • decreased keratinocyte apoptosis (MP:0009581)

  • decreased physiological sensitivity to xenobiotic (MP:0008874)
    • more 7 phenotypes
  • decreased response of heart to induced stress (MP:0004486)

  • decreased sensitivity to induced cell death (MP:0008944)

  • hypertension (MP:0000231)

  • increased incidence of tumors by chemical induction (MP:0004499)

  • increased systemic arterial systolic blood pressure (MP:0006144)

  • neoplasm (MP:0002006)

  • salt-sensitive hypertension (MP:0004217)
    • Detailed phenotype data

    Ordering Information

    供与核酸Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Pgk polyA, Jellyfish (Aequorea victoria) GFP cDNA, Bovine growth hormone PolyA signal, Mouse Map3k5 genomic DNA, SV40 nuclear localization signal sequence, E. coli beta-galactosidase, Mouse Map3k6 genomic DNA, P1 Phage loxP sites, Klebsiella pneumoniae Neomycin resistance gene, Mouse Map3k15 genomic DNA
    Research applicationApoptosis Research
    Cre/loxP system
    Fluorescent Proteins/lacZ System
    Immunology and Inflammation Research
    提供条件利用者は提供承諾書を用いて、事前に寄託者の承諾を得る。営利機関の利用希望者は事前に利用条件等につき、寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
    Depositor一條　秀憲（国立大学法人東京大学大学院）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data