Strain Data Sheet

RBRC05926

Strain Information

Image
BRC No.RBRC05926
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Map3k5<tm1Hijo> Map3k6<tm1Hijo>
Former Common nameASK1/2-DKO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Hidenori Ichijo, Graduate School of Pharmaceutical Sciences, The University of Tokyo. The double mutant mice were generated by crossing with Map3k5 KO mice and Map3k6 KO mice. C57BL/6J background.
Strain descriptionMap3k5 (ASK1) and Map3k6 (ASK2) genes double knockout mice. The coding region of Exon 1 of Map3k5 gene was replaced with a GFP/PGK-neo cassette (GFP expression was not observed). The exons 1 to 5 of Map3k6 were replaced with a NLS-lacZ cassette and a pgk-neo cassette. Double homozygous mutant mice are viable and fertile.
Colony maintenanceMap3k5: Homozygote x Homozygote; Map3k6: Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesEMBO J., 28, 843-853 (2009). 19214184
EMBO rep., 2, 222-228 (2001). 11266364
J. Biol. Chem., 282, 7522-7531 (2007). 17210579

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Map3k5mitogen-activated protein kinase kinase kinase 510Map3k5<tm1Hijo>targeted mutation 1, Hidenori Ichijo

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)10

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)10GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)10mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Map3k6mitogen-activated protein kinase kinase kinase 64Map3k6<tm1Hijo>targeted mutation 1, Hidenori Ichijo

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)4GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)4nls

Ordering Information

Donor DNAMouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Pgk polyA, Jellyfish (Aequorea victoria) GFP cDNA, Bovine Growth hormone polyA signal, Mouse Map3k5 genomic DNA, SV40 nuclear localization signal sequence, E. coli beta-galactosidase, Mouse Map3k6 genomic DNA
Research applicationApoptosis Research
Fluorescent Proteins/lacZ System
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorHidenori Ichijo (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data