Strain Data Sheet

RBRC05925

Strain Information

Image
BRC No.RBRC05925
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Smap2<tm1Sata>
Former Common nameSMAP2 KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearance
Strain developmenteveloped by Masanobu Satake, Institute of Development, Aging and Cancer, Tohoku University and Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionSmap2 gene knockout mice. Homozygous mutant males exhibit hypoplastic acrosome and are infertile due to globozoospermia.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl] Homozygous mutant males are infertile.
ReferencesMol. Biol. Cell, 17, 2592-2603 (2006). 16571680
Cell Struct. Funct., 36, 83-95 (2011). 21368446

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Smap2stromal membrane-associated GTPase-activating protein 24Smap2<tm1Sata>targeted mutation 1, Masanobu Satake

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4

Ordering Information

Donor DNAMouse SMAP2 genome, E. coli Beta-galactosidase, E. coli Neomycin resistance gene
Research applicationCell Biology Research
Developmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorMasanobu Satake (Tohoku University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data