Strain Data Sheet

RBRC05849

Strain Information

Image
BRC No.RBRC05849
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Dkk3<tm1.1>
Former Common nameC57BL/6J-Dkk3 KO(wt/KO/Cre), Dkk3 KO(wt/KO/Cre)
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Masaki Watabe and Hiromi Kumon, Okayama University Graduate School of Medicine and Kazuhiko Ochiai, Faculty of Veterinary Science, School of Veterinary Nursing and Technology. Bruce4 ES cells were used. Exon 5 to 6 and PGK-neo cassette were removed by crossing with a Cre mice (B6 background).
Strain descriptionREIC/Dkk3 gene knockout mice. Exons 5 and 6 were removed.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]Homozygous mutant mice are viable and fertile.
ReferencesActa Med. Okayama, 68(2):63-78 (2014). 24743782
Cancer Res., 68, 8333-8341 (2008). 18922905
Biochem. Biophys. Res. Commun., 268, 20-24 (2000). 10652205

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dkk3dickkopf homolog 3 (Xenopus laevis)7Dkk3<tm1.1>targeted mutation 1.1, Hiromi Kumon

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Ordering Information

Donor DNAPhage P1 loxP site, mouse Dkk3 genomic DNA (NCBI.No.BC046304)
Research applicationCancer Research
Cre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Acta Med. Okayama, 68(2):63-78 (2014).
DepositorHiromi Kumon (Momotaro-Gene Inc.)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data