Strain Data Sheet

RBRC05765

Strain Information

Image
BRC No.RBRC05765
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-H2az1<tm1.1Hko> H2az2<tm1.1Hko>
Former Common nameH2AZ1/2 floxed, B6-H2AZ1/2 floxed
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain development理化学研究所免疫 アレルギーセンター 古関明彦先生。M1 ES細胞を用いて作出。Neoは除去済み。
Strain descriptionH2A.Zのfloxedマウス。H2A.Z1/2は、哺乳類細胞のクロマチンに恒常的に存在するヒストン変異体。この遺伝子を欠損したマウスは胎生致死 (E6.5) となることから、哺乳類の発生、生存に必須なヒストンであると考えられる。クロマチン内でのエピジェネチックな遺伝子制御機構の解明に有用。
Colony maintenanceH2afz : Homozygote x Homozygote ; H2afv : Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesCurr. Biol., 11, 1183-1187 (2001). 11516949

Health Report

Examination Date / Room / Rack2024/02/26Room:4-BRack:E
2023/11/27Room:4-BRack:E
2023/08/30Room:4-BRack:E
2023/08/28Room:4-BRack:E
2023/05/29Room:4-BRack:E
2023/03/22Room:4-BRack:E
2023/02/27Room:4-BRack:E
2022/11/29Room:4-BRack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
H2az2H2A.Z histone variant 211H2az2<tm1.1Hko>targeted mutation 1.1, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
H2afzH2A.Z variant histone 13H2afz<tm1.1Hko>targeted mutation 1.1, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Ordering Information

供与核酸P1 Phage loxP sites, yeast FRT (flipase recombination target) site, mouse H2az1 genomic DNA, mouse H2az2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
提供条件条件を付加する。
利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
Depositor古関　明彦（独立行政法人理化学研究所）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
生体マウスを3~6ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Lowden C, Boulet A, Boehler NA, Seecharran S, Rios Garcia J, Lowe NJ, Liu J, Ong JLK, Wang W, Ma L, Cheng AH, Senatore A, Monks DA, Liu BH, Leary SC, Cheng HM.
Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survival.
Cell Rep 36(11) 109704(2021) 34525369

Rispal J, Baron L, Beaulieu JF, Chevillard-Briet M, Trouche D, Escaffit F.
The H2A.Z histone variant integrates Wnt signaling in intestinal epithelial homeostasis.
Nat Commun 10(1) 1827(2019) 31015444

Shen T, Ji F, Wang Y, Lei X, Zhang D, Jiao J.
Brain-specific deletion of histone variant H2A.z results in cortical neurogenesis defects and neurodevelopmental disorder.
Nucleic Acids Res 46(5) 2290-2307(2018) 29294103