Strain Data Sheet

RBRC05760

Strain Information

Image
BRC No.RBRC05760
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Spred1<tm1.1Ayos>
Former Common nameSpred1 floxed, B6-Spred1 floxed
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development慶應義塾大学医学部・吉村昭彦先生。Neoカセットはflp-mediated recombinationにより除去済み。
Strain descriptionSpred1遺伝子のfloxedマウス。エクソン7がloxP配列で挟まれている。
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesGenes Cells., 16, 803-814 (2011). 21668589

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Spred1sprouty protein with EVH-1 domain 1, related sequence2Spred1<tm1.1Ayos>targeted mutation 1.1, Akihiko Yoshimura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Ordering Information

供与核酸Bacteriophage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Spred1 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
FLP/frt system
Immunology and Inflammation Research
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Genes Cells., 16, 803-814 (2011).
研究成果の公表にあたって謝辞の表明を必要とする。
Depositor吉村 昭彦(慶應義塾大学)
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data