Strain Data Sheet

RBRC05745

Strain Information

Image
BRC No.RBRC05745
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(AS071-lacZ)A
Former Common nameAS071 Tg line A, B6-AS071-lacZ#A
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Norihiro Okada, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology in 2012. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. Three lines were generated : line A (RBRC05745), line B (RBRC05746), line C (RBRC05747) (main line).
Strain descriptionAS071-lacZ transgenic mice. AS071, one AmnSINE1 locus is a distal enhancer for Fgf8 expressed in the developing diencephalon. The expression pattern of lacZ driven by AS071 resembles that of diencephalic Fgf8 (dorsal midline, lateral wall and ventral midline of hypothalamus).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJmsSlc]
References
A SINE-derived element constitutes a unique modular enhancer for mammalian diencephalic Fgf8.
Nakanishi A, Kobayashi N, Suzuki-Hirano A, Nishihara H, Sasaki T, Hirakawa M, Sumiyama K, Shimogori T, Okada N
PLoS One, 7, e43785 (2012). 22937095

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
lacZbeta-galactosidase (E. coli)UNmouse Heat Shock Protein 68 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Heat Shock Protein 68 promoter, E. coli lacZ gene, SV40 PolyA signal, E. coli Ampicilin resistance gene, Mouse, AS071配列 (Chr19 : 45, 641, 837-45, 642, 422, NCBI37/mm9)
    Research applicationDevelopmental Biology Research
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 7, e43785 (2012). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
    DepositorNorihiro Okada (Tokyo Institute of Technology)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data