Strain Data Sheet

RBRC05737

Strain Information

Image
BRC No.RBRC05737
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(CAG-tdKaede)15Utr
Former Common nameWK-m15
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Dr. Yoshihiro Miwa, Graduate School of Comprehensive Human Sciences, University of Tsukuba in 2007. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The Kaede cDNA was provided by Dr. Atsushi Miyawaki, RIKEN Brain Science Institute. C57BL/6 background (RBRC05737), albino C57BL/6 background (RBRC05738).
Strain descriptionThese transgenic mice expressing a Kaede, photoconvertible fluorescence protein under the control of the CAG promoter. The expression of green fluoresce of Kaede mice can be converted to red on irradiation with UV or violet light.
Colony maintenanceCarrier x Carrier [Homozygote x Homozygote]
References
Tyrosinase subcellular distribution and kinetic parameters in wild type and C-locus mutant C57BL/6J mice.
D Townsend, C J Witkop, J Mattson
J. Exp. Zool., 216, 113-119 (1981). 6793688

Monitoring cellular movement in vivo with photoconvertible fluorescence protein "Kaede" transgenic mice.
Michio Tomura, Naoki Yoshida, Junko Tanaka, Satoshi Karasawa, Yoshihiro Miwa, Atsushi Miyawaki, Osami Kanagawa
Proc. Natl. Acad. Sci. USA, 105(31):10871-10876 (2008). 18663225

Health Report

Examination Date / Room / Rack2025/11/10Room:3-ARack:CSentinel mouse program
2025/09/01Room:3-ARack:CSentinel mouse program
2025/06/16Room:3-ARack:CSentinel mouse program
2025/04/07Room:3-ARack:CSentinel mouse program
2025/02/17Room:3-ARack:CAdditional tests
2025/01/14Room:3-ARack:CAdditional tests
2025/01/07Room:3-ARack:CSentinel mouse program
2024/10/07Room:3-ARack:CSentinel mouse program
2024/07/08Room:3-ARack:CSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
tdKaede Trachyphyllia geoffroy Kaede cDNAUNtdKaede CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
SV40 polyA siteUN

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • normal phenotype (MP:0002873)
  • Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Trachyphyllia geoffroy Kaede cDNA, SV40 Poly(A) site
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 105, 10871-10876 (2008). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.
    In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Kaede, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Proc. Natl. Acad. Sci. USA, 99(20) 12651–12656 (2002).
    DepositorYoshihiro Miwa (University of Tsukuba)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (1 to 3 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Aug 2016
    Mouse of the Month Dec 2020

    BRC mice in Publications

    Xing Y, Zhou Y, Wang R, Chen J, Yang L, Meng X, Wang J, Ouyang Q, Zhao J, Chen F, Saw PE, Fan J, Huang JD, Wu W, Liu Q, Song E, Huang D.
    Long-range deployment of tumor-antigen-specific cytotoxic T lymphocytes inhibits lung metastasis of breast cancer.
    Dev Cell 61(1) 117-132.e10(2026) 40876455
    Heim TA, Ibrahim O, Lin Z, Schultz AC, Steele MM, Mudianto T, Lund AW.
    CXCR6 promotes dermal CD8+ T cell survival and transition to long-term tissue residence.
    J Immunol 215(1) (2026) 40906897
    Raimondo MG, Mohammadian H, Angeli MR, Alivernini S, Fedorchenko V, Huang K, Demmler R, Rhein P, Xu C, Li YN, Micheroli R, Winter Z, Rigau AR, Anchang CG, Soare A, Luber M, Labinsky H, Chang J, Günther C, Fearon U, Veale DJ, Ciccia F, Rech J, Sticherling M, Bäuerle T, Distler JHW, Kurowska-Stolarska MS, Mack M, Ekici AB, Croft AP, Distler O, Maric HM, Ospelt C, Cañete JD, D'Agostino MA, Schett G, Rauber S, Ramming A.
    Skin-derived myeloid precursors and joint-resident fibroblasts spread psoriatic disease from skin to joints.
    Nat Immunol 27(1) 35-47(2026) 41482544
    Friedman DJ, Ramin S, Blair T, Kramer G, Bambina S, Alice AF, Baird J, Medler T, Young KH, Crittenden MR, Gough MJ.
    Fluorescence tracking Treg movement identifies anti-CCR8 and radiation as a therapeutic combination.
    iScience 29(2) 114572(2026) 41585478
    Lamorte S, Quevedo R, Jin R, Neufeld L, Liu ZQ, Ciudad MT, Lukhele S, Bruce J, Mishra S, Zhang X, Saeed ZK, Berman H, Philpott DJ, Girardin SE, Harding S, Munn DH, Mak TW, Karlsson MCI, Brooks DG, McGaha TL.
    Lymph node macrophages drive immune tolerance and resistance to cancer therapy by induction of the immune-regulatory cytokine IL-33.
    Cancer Cell (2025) 40054466
    Tanaka T, Sasaki N, Krisnanda A, Amin HZ, Ito K, Horibe S, Matsuo K, Hirata KI, Nakayama T, Rikitake Y.
    C-C chemokine receptor 4 deficiency exacerbates early atherosclerosis in mice.
    Elife 13 (2025) 40608058
    Nemoto Y, Morikawa R, Yonemoto Y, Tanaka S, Takei Y, Oshima S, Nagaishi T, Tsuchiya K, Nakamura T, Takenaka K, Ohtsuka K, Chen X, Okazawa H, Okamoto R, Watanabe M, von Andrian UH.
    Intestinal CD4-CD8αβ-TCRαβ+ T cells function as tolerogenic antigen presenting cells in mice.
    Nat Commun 16(1) 7072(2025) 40750764
    Roulstone V, Kyula-Currie J, Wright J, Patin EC, Dean I, Yu L, Barreiro-Alonso A, Melake M, Choudhary J, Elliott R, Lord CJ, Mansfield D, Matthews N, Chauhan R, Jennings V, Chan Wah Hak C, Baldock H, Butera F, Appleton E, Nenclares P, Pederson M, Foo S, Wongariyapak A, Rullan A, Tenev T, Meier P, Vile R, Pandha H, Melcher A, McLaughlin M, Harrington KJ.
    Palbociclib and dsRNA sensor co-operate to enhance anti-cancer effects through ER stress and modulation of immune evasion.
    Nat Commun 16(1) 4855(2025) 40413207
    Johansson A, Khalilnezhad A, Takizawa H, Mizuno H, Suda T, Umemoto T.
    Mobilization dynamics of bone marrow hematopoietic stem cells during hematopoietic regeneration.
    Exp Hematol 138 104281(2024) 39009278
    Wang G, Muñoz-Rojas AR, Spallanzani RG, Franklin RA, Benoist C, Mathis D.
    Adipose-tissue Treg cells restrain differentiation of stromal adipocyte precursors to promote insulin sensitivity and metabolic homeostasis.
    Immunity 57(6) 1345-1359.e5(2024) 38692280
    Wang H, Yao Z, Kang K, Zhou L, Xiu W, Sun J, Xie C, Yu M, Li Y, Zhang Y, Zheng Y, Lin G, Pan X, Wu Y, Luo R, Wang L, Tang M, Liao S, Zhu J, Zhou X, Zhang X, Xu Y, Liu Y, Peng F, Wang J, Xiang L, Yin L, Deng L, Huang M, Gong Y, Zou B, Wang H, Wu L, Yuan Z, Bi N, Fan M, Xu Y, Tong R, Yi L, Gan L, Xue J, Mo X, Chen C, Na F, Lu Y.
    Preclinical study and phase II trial of adapting low-dose radiotherapy to immunotherapy in small cell lung cancer.
    Med 5(10) 1237-1254.e9(2024) 38964333
    Miyamoto K, Sujino T, Harada Y, Ashida H, Yoshimatsu Y, Yonemoto Y, Nemoto Y, Tomura M, Melhem H, Niess JH, Suzuki T, Suzuki T, Suzuki S, Koda Y, Okamoto R, Mikami Y, Teratani T, Tanaka KF, Yoshimura A, Sato T, Kanai T.
    The gut microbiota-induced kynurenic acid recruits GPR35-positive macrophages to promote experimental encephalitis.
    Cell Rep 42(8) 113005(2023) 37590143
    Steele MM, Jaiswal A, Delclaux I, Dryg ID, Murugan D, Femel J, Son S, du Bois H, Hill C, Leachman SA, Chang YH, Coussens LM, Anandasabapathy N, Lund AW.
    T cell egress via lymphatic vessels is tuned by antigen encounter and limits tumor control.
    Nat Immunol 24(4) 664-675(2023) 36849745
    Prizant H, Patil N, Negatu S, Bala N, McGurk A, Leddon SA, Hughson A, McRae TD, Gao YR, Livingstone AM, Groom JR, Luster AD, Fowell DJ.
    CXCL10+ peripheral activation niches couple preferred sites of Th1 entry with optimal APC encounter.
    Cell Rep 36(6) 109523(2021) 34380032
    Sanmarco LM, Wheeler MA, Gutiérrez-Vázquez C, Polonio CM, Linnerbauer M, Pinho-Ribeiro FA, Li Z, Giovannoni F, Batterman KV, Scalisi G, Zandee SEJ, Heck ES, Alsuwailm M, Rosene DL, Becher B, Chiu IM, Prat A, Quintana FJ.
    Gut-licensed IFNγ+ NK cells drive LAMP1+TRAIL+ anti-inflammatory astrocytes.
    Nature 590(7846) 473-479(2021) 33408417
    Kitamoto S, Nagao-Kitamoto H, Jiao Y, Gillilland MG 3rd, Hayashi A, Imai J, Sugihara K, Miyoshi M, Brazil JC, Kuffa P, Hill BD, Rizvi SM, Wen F, Bishu S, Inohara N, Eaton KA, Nusrat A, Lei YL, Giannobile WV, Kamada N.
    The Intermucosal Connection between the Mouth and Gut in Commensal Pathobiont-Driven Colitis.
    Cell 182(2) 447-462.e14(2020) 32758418
    Lee KC, Chen P, Maricic I, Inamine T, Hu J, Gong S, Sun JC, Dasgupta S, Lin HC, Lin YT, Loomba R, Stärkel P, Kumar V, Schnabl B.
    Intestinal iNKT cells migrate to liver and contribute to hepatocyte apoptosis during alcoholic liver disease.
    Am J Physiol Gastrointest Liver Physiol 316(5) G585-G597(2019) 30817180
    Nakamura YK, Janowitz C, Metea C, Asquith M, Karstens L, Rosenbaum JT, Lin P.
    Short chain fatty acids ameliorate immune-mediated uveitis partially by altering migration of lymphocytes from the intestine.
    Sci Rep 7(1) 11745(2017) 28924192