Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC05737 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6.Cg-Tg(CAG-tdKaede)15Utr |
| Former Common name | WK-m15 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6JJcl |
| Appearance | |
| Strain development | Developed by Dr. Yoshihiro Miwa, Graduate School of Comprehensive Human Sciences, University of Tsukuba in 2007. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The Kaede cDNA was provided by Dr. Atsushi Miyawaki, RIKEN Brain Science Institute. C57BL/6 background (RBRC05737), albino C57BL/6 background (RBRC05738). |
| Strain description | These transgenic mice expressing a Kaede, photoconvertible fluorescence protein under the control of the CAG promoter. The expression of green fluoresce of Kaede mice can be converted to red on irradiation with UV or violet light. |
| Colony maintenance | Carrier x Carrier [Homozygote x Homozygote] |
| References | Tyrosinase subcellular distribution and kinetic parameters in wild type and C-locus mutant C57BL/6J mice. D Townsend, C J Witkop, J Mattson J. Exp. Zool., 216, 113-119 (1981). 6793688Monitoring cellular movement in vivo with photoconvertible fluorescence protein "Kaede" transgenic mice. Michio Tomura, Naoki Yoshida, Junko Tanaka, Satoshi Karasawa, Yoshihiro Miwa, Atsushi Miyawaki, Osami Kanagawa Proc. Natl. Acad. Sci. USA, 105(31):10871-10876 (2008). 18663225 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2025/11/10Room:3-ARack:CSentinel mouse program 2025/09/01Room:3-ARack:CSentinel mouse program 2025/06/16Room:3-ARack:CSentinel mouse program 2025/04/07Room:3-ARack:CSentinel mouse program 2025/02/17Room:3-ARack:CAdditional tests 2025/01/14Room:3-ARack:CAdditional tests 2025/01/07Room:3-ARack:CSentinel mouse program 2024/10/07Room:3-ARack:CSentinel mouse program 2024/07/08Room:3-ARack:CSentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| tdKaede | Trachyphyllia geoffroy Kaede cDNA | UN | tdKaede | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
| SV40 polyA site | UN | ||||||
Phenotype | |
|---|---|
| Phenotype annotation from literatures by Mammalian phenotype ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Trachyphyllia geoffroy Kaede cDNA, SV40 Poly(A) site |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 105, 10871-10876 (2008). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Kaede, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Proc. Natl. Acad. Sci. USA, 99(20) 12651–12656 (2002). |
| Depositor | Yoshihiro Miwa (University of Tsukuba) |
| Strain Status | Live mice Frozen embryos Frozen sperm |
| Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Aug 2016 Mouse of the Month Dec 2020 |
BRC mice in Publications |
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Heim TA, Ibrahim O, Lin Z, Schultz AC, Steele MM, Mudianto T, Lund AW. CXCR6 promotes dermal CD8+ T cell survival and transition to long-term tissue residence. J Immunol 215(1) (2026) 40906897 |
Raimondo MG, Mohammadian H, Angeli MR, Alivernini S, Fedorchenko V, Huang K, Demmler R, Rhein P, Xu C, Li YN, Micheroli R, Winter Z, Rigau AR, Anchang CG, Soare A, Luber M, Labinsky H, Chang J, Günther C, Fearon U, Veale DJ, Ciccia F, Rech J, Sticherling M, Bäuerle T, Distler JHW, Kurowska-Stolarska MS, Mack M, Ekici AB, Croft AP, Distler O, Maric HM, Ospelt C, Cañete JD, D'Agostino MA, Schett G, Rauber S, Ramming A. Skin-derived myeloid precursors and joint-resident fibroblasts spread psoriatic disease from skin to joints. Nat Immunol 27(1) 35-47(2026) 41482544 |
Friedman DJ, Ramin S, Blair T, Kramer G, Bambina S, Alice AF, Baird J, Medler T, Young KH, Crittenden MR, Gough MJ. Fluorescence tracking Treg movement identifies anti-CCR8 and radiation as a therapeutic combination. iScience 29(2) 114572(2026) 41585478 |
Lamorte S, Quevedo R, Jin R, Neufeld L, Liu ZQ, Ciudad MT, Lukhele S, Bruce J, Mishra S, Zhang X, Saeed ZK, Berman H, Philpott DJ, Girardin SE, Harding S, Munn DH, Mak TW, Karlsson MCI, Brooks DG, McGaha TL. Lymph node macrophages drive immune tolerance and resistance to cancer therapy by induction of the immune-regulatory cytokine IL-33. Cancer Cell (2025) 40054466 |
Tanaka T, Sasaki N, Krisnanda A, Amin HZ, Ito K, Horibe S, Matsuo K, Hirata KI, Nakayama T, Rikitake Y. C-C chemokine receptor 4 deficiency exacerbates early atherosclerosis in mice. Elife 13 (2025) 40608058 |
Nemoto Y, Morikawa R, Yonemoto Y, Tanaka S, Takei Y, Oshima S, Nagaishi T, Tsuchiya K, Nakamura T, Takenaka K, Ohtsuka K, Chen X, Okazawa H, Okamoto R, Watanabe M, von Andrian UH. Intestinal CD4-CD8αβ-TCRαβ+ T cells function as tolerogenic antigen presenting cells in mice. Nat Commun 16(1) 7072(2025) 40750764 |
Roulstone V, Kyula-Currie J, Wright J, Patin EC, Dean I, Yu L, Barreiro-Alonso A, Melake M, Choudhary J, Elliott R, Lord CJ, Mansfield D, Matthews N, Chauhan R, Jennings V, Chan Wah Hak C, Baldock H, Butera F, Appleton E, Nenclares P, Pederson M, Foo S, Wongariyapak A, Rullan A, Tenev T, Meier P, Vile R, Pandha H, Melcher A, McLaughlin M, Harrington KJ. Palbociclib and dsRNA sensor co-operate to enhance anti-cancer effects through ER stress and modulation of immune evasion. Nat Commun 16(1) 4855(2025) 40413207 |
Johansson A, Khalilnezhad A, Takizawa H, Mizuno H, Suda T, Umemoto T. Mobilization dynamics of bone marrow hematopoietic stem cells during hematopoietic regeneration. Exp Hematol 138 104281(2024) 39009278 |
Wang G, Muñoz-Rojas AR, Spallanzani RG, Franklin RA, Benoist C, Mathis D. Adipose-tissue Treg cells restrain differentiation of stromal adipocyte precursors to promote insulin sensitivity and metabolic homeostasis. Immunity 57(6) 1345-1359.e5(2024) 38692280 |
Wang H, Yao Z, Kang K, Zhou L, Xiu W, Sun J, Xie C, Yu M, Li Y, Zhang Y, Zheng Y, Lin G, Pan X, Wu Y, Luo R, Wang L, Tang M, Liao S, Zhu J, Zhou X, Zhang X, Xu Y, Liu Y, Peng F, Wang J, Xiang L, Yin L, Deng L, Huang M, Gong Y, Zou B, Wang H, Wu L, Yuan Z, Bi N, Fan M, Xu Y, Tong R, Yi L, Gan L, Xue J, Mo X, Chen C, Na F, Lu Y. Preclinical study and phase II trial of adapting low-dose radiotherapy to immunotherapy in small cell lung cancer. Med 5(10) 1237-1254.e9(2024) 38964333 |
Miyamoto K, Sujino T, Harada Y, Ashida H, Yoshimatsu Y, Yonemoto Y, Nemoto Y, Tomura M, Melhem H, Niess JH, Suzuki T, Suzuki T, Suzuki S, Koda Y, Okamoto R, Mikami Y, Teratani T, Tanaka KF, Yoshimura A, Sato T, Kanai T. The gut microbiota-induced kynurenic acid recruits GPR35-positive macrophages to promote experimental encephalitis. Cell Rep 42(8) 113005(2023) 37590143 |
Steele MM, Jaiswal A, Delclaux I, Dryg ID, Murugan D, Femel J, Son S, du Bois H, Hill C, Leachman SA, Chang YH, Coussens LM, Anandasabapathy N, Lund AW. T cell egress via lymphatic vessels is tuned by antigen encounter and limits tumor control. Nat Immunol 24(4) 664-675(2023) 36849745 |
Prizant H, Patil N, Negatu S, Bala N, McGurk A, Leddon SA, Hughson A, McRae TD, Gao YR, Livingstone AM, Groom JR, Luster AD, Fowell DJ. CXCL10+ peripheral activation niches couple preferred sites of Th1 entry with optimal APC encounter. Cell Rep 36(6) 109523(2021) 34380032 |
Sanmarco LM, Wheeler MA, Gutiérrez-Vázquez C, Polonio CM, Linnerbauer M, Pinho-Ribeiro FA, Li Z, Giovannoni F, Batterman KV, Scalisi G, Zandee SEJ, Heck ES, Alsuwailm M, Rosene DL, Becher B, Chiu IM, Prat A, Quintana FJ. Gut-licensed IFNγ+ NK cells drive LAMP1+TRAIL+ anti-inflammatory astrocytes. Nature 590(7846) 473-479(2021) 33408417 |
Kitamoto S, Nagao-Kitamoto H, Jiao Y, Gillilland MG 3rd, Hayashi A, Imai J, Sugihara K, Miyoshi M, Brazil JC, Kuffa P, Hill BD, Rizvi SM, Wen F, Bishu S, Inohara N, Eaton KA, Nusrat A, Lei YL, Giannobile WV, Kamada N. The Intermucosal Connection between the Mouth and Gut in Commensal Pathobiont-Driven Colitis. Cell 182(2) 447-462.e14(2020) 32758418 |
Lee KC, Chen P, Maricic I, Inamine T, Hu J, Gong S, Sun JC, Dasgupta S, Lin HC, Lin YT, Loomba R, Stärkel P, Kumar V, Schnabl B. Intestinal iNKT cells migrate to liver and contribute to hepatocyte apoptosis during alcoholic liver disease. Am J Physiol Gastrointest Liver Physiol 316(5) G585-G597(2019) 30817180 |
Nakamura YK, Janowitz C, Metea C, Asquith M, Karstens L, Rosenbaum JT, Lin P. Short chain fatty acids ameliorate immune-mediated uveitis partially by altering migration of lymphocytes from the intestine. Sci Rep 7(1) 11745(2017) 28924192 |