Strain Data Sheet

RBRC05735

Strain Information

Image
BRC No.RBRC05735
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Izumo3<tm1Osb>
Former Common nameIzumo3 KO
H-2 Haplotype
ES Cell line
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Naokazu Inoue, Research Institute for Microbial Diseases, Osaka University in 2012. C57BL/6 and DBA/2 mixed background.
Strain descriptionIzumo3 knockout mice. Homozygous mutant males have a tendency to exhibit infertility.
Colony maintenanceHeterozygote x Wild-type [or Crossing to Jcl : B6D2F1]
ReferencesMol. Reprod. Dev., (2021). doi: 10.1002/mrd.23520. 34114279

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Izumo3IZUMO family member 34Izumo3 <tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site4Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4neo; neomycin;human phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAyeast FRT (flipase recombination target) site, Phage P1 loxP site, human PGK promoter, E. coli Neomycin resistance gene, human PGK poly (A), mouse Izumo3 genomic DNA
Research applicationDevelopmental Biology Research
Immunology and Inflammation Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. Mol. Reprod. Dev., (2021). doi: 10.1002/mrd.23520.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorNaokazu Inoue (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data