Strain Information | |
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Image | |
BRC No. | RBRC05733 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | STOCK Dcst1/Dcst2<tm1Osb> |
Former Common name | Dcst1 KO |
H-2 Haplotype | |
ES Cell line | |
Background strain | Jcl:B6D2F1 |
Appearance | |
Strain development | Developed by Naokazu Inoue, Research Institute for Microbial Diseases, Osaka University in 2012. C57BL/6 and DBA/2 mixed background. |
Strain description | Dcst1 gene knockout mice. Homozygous mutant males are infertile. |
Colony maintenance | Heterozygote x Wild-type [or Crossing to Jcl : B6D2F1] |
References | Elife, 10:e66313 (2021). 33871360 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Dcst1DC-STAMP domain containing 13Dcst1 <tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Dcst2DC-STAMP domain containing 23Dcst2<tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site3Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)3neo; neomycin;human phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | yeast FRT (flipase recombination target) sites, Phage P1 loxP sites, human PGK promoter, E. coli Neomycin resistance gene, human PGK poly (A), mouse Dcst1 genomic DNA, mouse Dcst2 genomic DNA |
Research application | Cre/loxP system Developmental Biology Research FLP/frt system |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Elife. 2021 Apr 19;10:e66313.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Naokazu Inoue (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |