RBRC05721

Information1

Image
BRC No.RBRC05721
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Oosp1<tm1a(KOMP)Osb>/90Osb
Former Common nameOosp1 floxed#90
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain
Appearance
Strain developmentDeveloped by Yoshitaka Fujihara and Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2011.
Strain descriptionOosp1 floxed mice. Exon 2 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [C57BL/6NJcl]
ReferencesCells. 2020 Mar 28;9(4). pii: E821. 32231122

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Oosp1oocyte secreted protein 119Oosp1 <tm1a(KOMP)Osb>targeted mutation 1a, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele namePromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)19En2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site19Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)19

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)19

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP19loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)19human beta-actin promoter

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal19

Information2

Donor DNAEn2 splice acceptor (mouse En2 intron 2 / exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli LacZ, SV40 polyadenylation site, hBactP (human beta-actin promoter), E. coli neomycin phosphotransferase (neoR) gene, yeast FRT (flippase recombination target) sites, phage P1 loxP, mouse Oosp1 genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cells. 2020 Mar 28;9(4). pii: E821.Using the BIOLOGICAL RESOURCE is limited to the academic research of academic institutions. The RECIPIENT must contact in advance the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Lab HP

BRC mice in Publications

No Data