Strain Data Sheet

RBRC05720

Strain Information

Image
BRC No.RBRC05720
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;BDF1-Oosp1<tm1a(KOMP)Osb>/30Osb
Former Common nameOosp1 floxed#30
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain
Appearance
Strain developmentDeveloped by Yoshitaka Fujihara and Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2011.
Strain descriptionOosp1 floxed mice. Exon 2 was flanked by loxP sites.
Colony maintenanceHomozygote x Homozygote [C57BL/6NJcl]
References
CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice.
Abbasi F, Kodani M, Emori C, Kiyozumi D, Mori M, Fujihara Y, Ikawa M
Cells, 9(4):821 (2020). 32231122

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
En2 En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)19En2
Frt yeast FRT (flippase recombination target) site19Frt
IRES internal ribosomal entry site (EMCV)19
Oosp1
MGI:2149290		oocyte secreted protein 119Oosp1 <tm1a(KOMP)Osb> targeted mutation 1a, Research Institute for Microbial Diseases, Osaka University
lacZ beta-galactosidase (E. coli)19
loxP phage P1 loxP19loxP
loxP phage P1 loxP19loxP
neo neomycin resistance gene (E. coli)19

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEn2 splice acceptor (mouse En2 intron 2 / exon 3 splice acceptor sequence), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli LacZ, SV40 polyadenylation site, hBactP (human beta-actin promoter), E. coli neomycin phosphotransferase (neoR) gene, yeast FRT (flippase recombination target) sites, phage P1 loxP, mouse Oosp1 genomic DNA
    Research applicationCre/loxP system
    Developmental Biology Research
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cells. 2020 Mar 28;9(4). pii: E821.Using the BIOLOGICAL RESOURCE is limited to the academic research of academic institutions. The RECIPIENT must contact in advance the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.　The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
    DepositorMasaru Okabe (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP

    BRC mice in Publications

    No Data