Strain Data Sheet

RBRC05659

Strain Information

Image
BRC No.RBRC05659
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ly75<tm1.1Ksat>
Former Common nameCd205<dtr>, B6- Ly75/DT-GFP KI
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain development理化学研究所免疫・アレルギー科学総合研究センター・佐藤克明先生 (2009)。B6.Cg-Thy1<a>由来のBruce 4 ES細胞由来。C57BL/6Jマウスに戻し交配 (9世代以上)。
Strain descriptionCD205 (Ly75) 遺伝子の3’UTRにIRES-DTR(diphtheria toxin receptor)-EGFP cassetteをノックインしたマウス。diphtheria toxin (DT) の投与により、CD205陽性通常型樹状細胞 (conventional dendritic cells ; cDCs) 特異的欠損マウスとしての利用可能。CD205陽性cDCsの生体内機能解析に有用。
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
ReferencesProc. Natl. Acad. Sci. USA, 109, 11288-11293 (2012). 22736794

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ly75lymphocyte antigen 752Ly75 <tm1.1Ksat>targeted mutation 1.1, Katsuaki Sato

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)2EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBEGFheparin-binding EGF-like growth factor (human)2HBEGF

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRES2internal ribosomal entry site (EMCV)2IRES2internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Ordering Information

供与核酸Encephalomyocarditis virus (EMCV) internal ribosomal entry site 2 (ires2), human diphtheria toxin receptor (DTR) corresponds to the heparin binding EGF-like growth factor, Aequorea Victoria EGFP cDNA, Phage P1 loxP site, mouse Ly75 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Immunology and Inflammation Research
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Proc. Natl. Acad. Sci. U S A, 109, 11288-11293 (2012).
非営利機関の利用に限る。利用者は事前に寄託者（佐藤克明 博士）の承諾（英文の「Approval form」）を得る。利用者は別途、「MTA for Siglech-IRES2DTREGFP Knock-In Micefor Academic Research Purposes」を提出する。
Depositor佐藤　克明（独立行政法人理化学研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. MTA for Cd205-IRES2DTREGFP Knock-In Mice for Academic Research Purposes (English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Depositor's present address:Division of Immunology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki.
Genotyping protocol -PCR-

BRC mice in Publications

No Data