Strain Data Sheet

RBRC05658

Strain Information

Image
BRC No.RBRC05658
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Siglech<tm1.1Ksat>
Former Common nameSiglech<dtr>, B6-Siglech/DT-GFP KI
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain development理化学研究所免疫・アレルギー科学総合研究センター・佐藤克明先生 (2009)。B6.Cg-Thy1<a>由来のBruce 4 ES細胞由来。C57BL/6Jマウスに戻し交配 (9世代以上)。
Strain description形質細胞様樹状細胞 (plasmacytoid dendritic cells ; pDCs) の特異的発現分子であるSiglech, sialic acid binding Ig-like lectin (Siglec)-H遺伝子の3’UTRにIRES-DTR (diphtheria toxin receptor)-EGFP cassetteをノックインしたマウス。diphtheria toxin (DT) 非投与ではSiglec-H欠損マウス、DT投与ではpDC特異的欠失マウスとしての利用が可能。pDCsの生体内機能解析に有用。
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
ReferencesImmunity, 35, 958-971 (2011). 22177923
J. Allergy Clin. Immunol., (2018). 29477579

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Siglechsialic acid binding Ig-like lectin H7Siglech<tm1.1Ksat>targeted mutation 1.1, Katsuaki Sato

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)7EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBEGFheparin-binding EGF-like growth factor (human)7HBEGF

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRES2internal ribosomal entry site (EMCV)7IRES2internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Ordering Information

供与核酸Encephalomyocarditis virus (EMCV) internal ribosomal entry site 2 (ires2), human diphtheria toxin receptor (DTR) corresponds to the heparin binding EGF-like growth factor, Aequorea Victoria EGFP, Phage P1 loxP site, mouse Siglech genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Immunology and Inflammation Research
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Immunity, 35, 958-971 (2011).
非営利機関の利用に限る。利用者は事前に寄託者（佐藤克明 博士）の承諾（英文の「Approval form」）を得る。利用者は別途、「MTA for Siglech-IRES2DTREGFP Knock-In Micefor Academic Research Purposes」を提出する。
Depositor佐藤　克明（独立行政法人理化学研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. MTA for Siglech-IRES2DTREGFP Knock-In Mice for Academic Research Purposes (English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Depositor's present address:Division of Immunology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki.
Genotyping protocol -PCR-

BRC mice in Publications

Konishi H, Okamoto T, Hara Y, Komine O, Tamada H, Maeda M, Osako F, Kobayashi M, Nishiyama A, Kataoka Y, Takai T, Udagawa N, Jung S, Ozato K, Tamura T, Tsuda M, Yamanaka K, Ogi T, Sato K, Kiyama H.
Astrocytic phagocytosis is a compensatory mechanism for microglial dysfunction.
EMBO J 39(22) e104464(2020) 32959911

Koda Y, Nakamoto N, Chu PS, Ugamura A, Mikami Y, Teratani T, Tsujikawa H, Shiba S, Taniki N, Sujino T, Miyamoto K, Suzuki T, Yamaguchi A, Morikawa R, Sato K, Sakamoto M, Yoshimoto T, Kanai T.
Plasmacytoid dendritic cells protect against immune-mediated acute liver injury via IL-35.
J Clin Invest 129(8) 3201-3213(2019) 31264967

Li C, Konishi H, Nishiwaki K, Sato K, Miyata T, Kiyama H.
A mouse model of microglia-specific ablation in the embryonic central nervous system.
Neurosci Res 173 54-61(2021) 34157360