Strain Data Sheet
RBRC05551
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC05551 |
| Type | Targeted Mutation and Transgene |
| Species | Mus musculus |
| Strain name | B6.Cg-Tg(Aicda-cre)9Mbu Gt(ROSA)26Sor<tm1Hjf> |
| Former Common name | Aicda-cre/rosa-tdRFP, B6-AicdaCre/ROSA26-tdRFP |
| H-2 Haplotype | |
| ES Cell line | AK7 [129S4/SvJaeSor] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Hitoshi Nagaoka, Graduate School of Medicine, Kyoto University. Rosa-tdRFP : Hans Jorg Fehling, Institute of Immunology, University Clinics Ulm. Aicda-cre : Meinrad Busslinger. Research Institute of Molecular Pathology. |
| Strain description | This strain has AID-Tg (Tg(Aicda-cre)9Mbu) and ROSA26 tdRFP reporter (Gt(ROSA)26Sor<tm1Hjf>) alleles. |
| Colony maintenance | Tg : Carrier x Carrier ; ROSA26 : Homozygote x Homozygote |
| References | Faithful activation of an extra-bright red fluorescent protein in "knock-in" Cre-reporter mice ideally suited for lineage tracing studies. Luche H, Weber O, Nageswara Rao T, Blum C, Fehling H J Eur. J. Immunol., 37, 43-53 (2007). 17171761Instructive role of the transcription factor E2A in early B lymphopoiesis and germinal center B cell development. Kwon K, Hutter C, Sun Q, Bilic I, Cobaleda C, Malin S, Busslinger M Immunity, 28, 751-762 (2008). 18538592Activation-induced cytidine deaminase expression in CD4+ T cells is associated with a unique IL-10-producing subset that increases with age. Qin H, Suzuki K, Nakata M, Chikuma S, Izumi N, Huong le T, Maruya M, Fagarasan S, Busslinger M, Honjo T, Nagaoka H PLoS One, 6, e29141 (2011). 22216188 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| GH | Growth hormone polyA (Bovine) | 6 | GH | ||||
| Gt(ROSA)26Sor MGI:104735 | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor<tm1Hjf> MGI:3696099 | targeted mutation 1, Hans Jorg Fehling | |||
| RFP | Red Fluorescent Protein (Discosoma sp.) | 6 | RFP | ||||
| SA | Splice acceptor (adenovirus) | 6 | SA | ||||
| lox2272 | phage P1 loxP variant | 6 | lox2272 | ||||
| lox2272 | phage P1 loxP variant | 6 | lox2272 | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| CD2 | CD2 (human) | UN | CD2 | ||||
| Ires | internal ribosomal entry site (EMCV) | UN | |||||
| cre | Phage P1 Cre recombinase | UN | cre | mouse Aicda promoter | |||
| nls | SV40 large T antigen nuclear localization signal (NLS) | UN | nls | ||||
| SV40 polyA signal | UN | ||||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | mouse Aicda genomic DNA (BAC clone RP24-68I7), SV40 nuclear localization sequence, Phage P1 Cre, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), human CD2 cDNA, SV40 polyadenylation site, yeast FRT (flipase recombination target) site, adenovirus splice acceptor site (SA), P1 phage loxP sites, E. coli neo, bovine growth hormone gene polyadenylation site, Discosoma sp. tdRFP cDNA, SV40 polyadenylation site, mouse ROSA26 locus genomic DNA |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 6, e29141 (2011). The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. The BIOLOGICAL RESOURCE contains Tg(Aicda-cre)9Mbu and Gt(ROSA)26Sortm1Hjf. Before executing the MTA between RIKEN BRC andthe RECIPIENT, the RECIPIENT shall obtain the prior written consent from two developer scientists; Dr. Meinrad Busslinger(Research Institute of Molecular Pathology) to use Tg(Aicda-cre)9Mbu and Dr. Hans Joerg Fehling, Institute of Immunology, University Clinics Ulm to use Gt(ROSA)26Sortm1Hjf. |
| Depositor | Tasuku Honjo (Kyoto University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
|---|
| No Data |