Strain Data Sheet

RBRC05544

Strain Information

Image
BRC No.RBRC05544
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S4-Tecpr1<tm1Chsa>
Former Common nameB6-Tecpr1 KO
H-2 Haplotype
ES Cell lineJ1 [129S4/SvJae]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Michinaga Ogawa, The Institute of Medical Science, The University of Tokyo.Knockout mice were generated by using J1 ES cells (129S4/SvJae derived). The mutant mice were backcrossed to C57BL/6JJcl.
Strain descriptionTecpr1, tectonin beta-propeller repeat containing 1 gene knockout mice. A neomycin selection cassette was inserted into the exon 2-6 of the Tecpr1 gene.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesMol. Cell, 45, 629-641 (2012). 22342342
Cell Host Microbe, 9, 376-389 (2011). 21575909

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tecpr1tectonin beta-propeller repeat containing 15Tecpr1 <tm1Chsa>targeted mutation 1, Chihiro Sasakawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (Klebsiella pneumoniae)5neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse phosphoglycerate kinase promoter (Pgk promoter), Klebsiella pneumoniae neomycin resistance gene, mouse Tecpr1 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Host Microbe, 9, 376-389 (2011). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorDrs. Chihiro Sasakawa and Michinaga Ogawa (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data