Strain Information | |
---|---|
Image | |
BRC No. | RBRC05543 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;B6129-Rest<tm1.1Yasu> |
Former Common name | Rest cKO, B6-Rest floxed |
H-2 Haplotype | |
ES Cell line | V6.5 [(C57BL/6J x 129S4/SvJae)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Yasuhiro Yamada, Gifu University Graduate School of Medicine / Center for iPS Cell Research and Application, Kyoto University. C57BL/6J and 129S4 mixed background. The neomycin resistant cassette was removed by Cre mediated recombination. |
Strain description | Rest, RE1-silencing transcription factor gene floxed mice. Exon 4 of Rest gene was replaced with a loxP-exon 4-IRES-GFP-loxP construct. Conditional Rest deficient mice can be generated by crossing with tissue-specific Cre mice. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Cell Stem Cell, 6, 10-15 (2010). 20085738 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GFPGreen Fluorescent Protein (Aequorea victoria)5 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)5internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP |
Ordering Information | |
---|---|
Donor DNA | Bacteriophage P1 loxP sites, Encephalomyocarditis virus IRES, A. victoria GFP cDNA, mouse Rest genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR, KYOTO UNIVERSITY at cira-keiyaku@cira.kyoto-u.ac.jp. |
Depositor | Yasuhiro Yamada (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |