Strain Data Sheet

RBRC05539

Strain Information

Image
BRC No.RBRC05539
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Gt(ROSA)26Sor<tm1(Stop-Bmi1,EGFP)>
Former Common nameR26Stop<FL>Bmi1, B6-ROSA26/Stop-Bmi1,GFP KI
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain development2009年 理化学研究所横浜免疫アレルギー研究所の古関 明彦先生と千葉大学岩間先生研究室との共同研究で作製した。R1 ES (Hybrid of two 129 substrains (129X1/SvJ and 129S1/SV-+<p>+<Tyr-c>Kitl<Sl-J>/+)。
Strain descriptionROSA26遺伝子座にloxP-STOP-loxP-FLAG-Bmi1-FRT-IRES-EGFP-FRTを挿入したノックインマウス。造血細胞特異的にBmi1を発現させると造血幹細胞活性が増殖する。
Colony maintenanceC57BL/6コンジェニック系統。C57BL/6NCr (日本SLC) に7世代以上の戻し交配を行う。その後，兄妹交配にて維持。交配によりY染色体はB6置換済み。
ReferencesPLoS One. 7(5):e36209 (2012). 22606246

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(Stop-Bmi1,EGFP)>

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bmi1Bmi1 polycomb ring finger oncogene6Bmi1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)6FLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)6internal ribosomal entry site from EMCV

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Ordering Information

供与核酸Bacteriophage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistance gene, STOP, FLAG epitope tag, mouse Bmi1 cDNA, yeast FRT (flipase recombination target) sites, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), jellyfish EGFP cDNA, Bovine PolyA signal, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。(will be announced.)
Depositor岩間　厚志（国立大学法人千葉大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data