Strain Data Sheet

RBRC05522

Strain Information

Image
BRC No.RBRC05522
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S7-Sox9<tm1(cre/ERT2)Haak>
Former Common nameSox9-CreERT2 KI, Sox9-CreERT2 knock-in, B6-Sox9 KI/CreER
H-2 Haplotype
ES Cell lineAB1 [129S7/SvEvBrd-Hprt<+>]
Background strain
Appearance
Strain developmentThe University of Texas MD Anderson Cancer Center・秋山治彦先生（2001）。C57BL/6背景。
Strain descriptionSox9 CreERノックインマウス。Sox9発現組織特異的にタモキシフェン誘導性Cre recombinaseを発現する。
Colony maintenanceHeterozygote x Wild-type [C57BL/6]
ReferencesGenesis., 48, 635-644 (2010). 20806356

Health Report

Examination Date / Room / Rack2024/01/22Room:3-CRack:G
2023/10/23Room:3-CRack:G
2023/07/24Room:3-CRack:G
2023/04/24Room:3-CRack:G
2023/01/23Room:3-CRack:G
2022/10/25Room:3-CRack:G
2022/07/25Room:3-CRack:G

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sox9SRY-box containing gene 911Sox9<tm1(cre/ERT2)Haak>targeted mutation 1, Haruhiko Akiyama

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)11ERT2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)11GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)11

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase11cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

供与核酸mouse Sox9 genomic DNA, EMCV internal ribosomal entry site (ires), bacteriophage P1 cre recombinase gene, human estrogen receptor binding domain (ERT2), mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, bovine polyA signal, yeast FRT (flipase recombination target) sites
Research applicationCre/loxP system
Developmental Biology Research
FLP/frt system
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Genesis., 48, 635-644 (2010).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Haruhiko Akiyama, Kyoto University, is requested. Depositor retains ownership of the Biological Resource, including any Biological Resource contained in any modification. Any Biological Resource delivered pursuant to the Agreement is understood to be experimental in nature and may have hazardous properties. The DEPOSITOR MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE BIOLOGICAL RESOURCE WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS. In no event shall Depositor be liable for any use by recipient of the Biological Resource for any loss, claim, damage or liability, of whatsoever kind of nature, which may arise from or in connection with the use, handling or storage of the Biological Resource. Except where limited by Federal law, or by the constitution and laws of the state governing the recipient, the recipient agrees to hold harmless Depositor and The University of Texas System and their Regents, officers, agents and employees, from any liability, loss or damage they may suffer as a result of claims, demands, costs or judgments against them arising out of recipient’s use of the Material and the use by recipient of the results obtained from the Research. The Biological Resource and modifications containing the Biological Resource may only be transferred to non-profit institutions for academic research purposes.
DepositorBenoit de Crombrugghe（The University of Texas M. D. Anderson Cancer Center）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
生体マウスを1~3ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data