Strain Data Sheet

RBRC05454

Strain Information

Image
BRC No.RBRC05454
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129B6(Cg)-Actb<tm1(tetO-ChR2*C128S/EYFP)Kftnk>
Former Common nametetO-ChR2 KI, B6-tetO-ChR2/YFP KI
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain development田中先生と山中先生が生理研で作成。G0G1 (阪大微研の岡部勝先生が樹立, 129とB6のhybrid) で作成。
Strain descriptiontetOプロモーターの制御下にchannelrhodopsin2 (C128S変異体) -EYFP融合タンパクを発現する。tetOカセットがbeta actin遺伝子下流にノックインされている。
Colony maintenanceB6Jと129S6のmixed background
ReferencesNat. Neurosci., 22(5):770-777 (2019). 30988523
Cell Rep., 36(4):109427(2021). 34320360
Cell Rep., 2(2):397-406 (2012). 22854021

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Actbactin, beta5Actb <<tm1(tetO-ChR2*C128S/EYFP)Kftnk>>targeted mutation 1, Kenji Tanaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ChR2channelrhodopsin-2 (Chlamydomonas reinhardtii)5ChR2E. coli Tetracycline operator, CMV minimal promoter, rabbit Beta globin intron

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)5EYFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal5

Ordering Information

供与核酸Phage P1 loxP sites, E. coli Tetracycline operator site, Cytomegalovirus CMV minimum promoter, Rabbit Beta globin intron, Chlamydomonas Channelrhodopsin-2, jellyfish EYFP cDNA, SV40 polyA signal, yeast FRT (flipase recombination target) site, mouse beta actin genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Tet system
提供条件条件を付加する。
利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。Cell Rep., 2(2):397-406 (2012).。学術機関の学術研究に限る。 利用者は提供承諾書を用いて、事前に寄託者へプロジェクト内容を通知する。学術論文へ発表する際は、最初の論文に限り寄託者を共著とする。
Depositor田中　謙二（慶應義塾大学）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Apr 2013

BRC mice in Publications

Abe Y, Kwon S, Oishi M, Unekawa M, Takata N, Seki F, Koyama R, Abe M, Sakimura K, Masamoto K, Tomita Y, Okano H, Mushiake H, Tanaka KF.
Optical manipulation of local cerebral blood flow in the deep brain of freely moving mice.
Cell Rep 36(4) 109427(2021) 34320360

Oishi M, Passlick S, Yamazaki Y, Unekawa M, Adachi R, Yamada M, Imayoshi I, Abe Y, Steinhäuser C, Tanaka KF.
Separate optogenetic manipulation of Nerve/glial antigen 2 (NG2) glia and mural cells using the NG2 promoter.
Glia 71(2) 317-333(2023) 36165697

Kato T, Mitsukura Y, Yoshida K, Mimura M, Takata N, Tanaka KF.
Oscillatory population-level activity of dorsal raphe serotonergic neurons is inscribed in sleep structure.
J Neurosci 42(38) 7244-55(2022) 35970565

Yamazaki Y, Abe Y, Fujii S, Tanaka KF.
Oligodendrocytic Na+-K+-Cl- co-transporter 1 activity facilitates axonal conduction and restores plasticity in the adult mouse brain.
Nat Commun 12(1) 5146(2021) 34446732

Kono A, Shikano Y, Tanaka KF, Yamaura K, Tsutsui-Kimura I.
Inhibition of the dorsomedial striatal direct pathway is essential for the execution of action sequences.
Neuropsychopharmacol Rep 43(3) 414-424(2023) 37553985

Murata J, Unekawa M, Kudo Y, Kotani M, Kanno I, Izawa Y, Tomita Y, Tanaka KF, Nakahara J, Masamoto K.
Acceleration of the Development of Microcirculation Embolism in the Brain due to Capillary Narrowing.
Stroke 54(8) 2135-2144(2023) 37309687