Strain Data Sheet
RBRC05450
Strain Information | |
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| Image | |
| BRC No. | RBRC05450 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6;CBB6(129)-Tg(Mlc1-tTA)2Rhn |
| Former Common name | Mlc1-tTA#2, B6-Mlc1-tTA Tg#2 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Kenji Tanaka, Columbia University in 2007. C57BL/6 and 129S6 mixed background. |
| Strain description | Mlc1-tTA transgenic mice. These transgenic mice express tetracycline-controlled transactivator (tTA) gene under the control of the mouse Mlc1 promoter. tTA expression is detected in Mlc1 positive astrocytes. |
| Colony maintenance | Carrier x Noncarrier [C57BL/6JJcl] |
| References | Flexible Accelerated STOP Tetracycline Operator-knockin (FAST): a versatile and efficient new gene modulating system. Kenji F Tanaka, Susanne E Ahmari, E David Leonardo, Jesse W Richardson-Jones, Elaine C Budreck, Peter Scheiffele, Shouta Sugio, Naoko Inamura, Kazuhiro Ikenaka, René Hen Biol. Psychiatry, 67, 770-773 (2010). 20163789 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| tTA | tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain) | UN | tTA | mouse Mlc1 promoter | |||
| SV40 polyA signal | UN | ||||||
Phenotype | |
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| Phenotype annotation from literatures by Mammalian phenotype ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | mouse Mlc1 genomic DNA, E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain, SV40 polyA signal |
| Research application | Tet system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Psychiatry, 67, 770-773 (2010). |
| Depositor | Kenji Tanaka (Keio University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Vittani M, Herlo R, Wang X, Christensen MDB, Vo CT, Mishima T, Kusk P, Konno A, Hirai H, Tsuboi T, Kitaguchi T, Kjaerby C, Asiminas A, Yokoyama T, Sakamoto M, Nedergaard M, Hirase H. Cerebral blood flow is modulated by astrocytic cAMP elevation independently of IP3R2-mediated Ca2+ signaling in mice. Proc Natl Acad Sci U S A 122(27) e2422069122(2025) 40591593 |
Balachandar L, Moncion C, Suarez A, Diaz JR. Characterization of Optimal Optogenetic Stimulation Paradigms to Evoke Calcium Events in Cortical Astrocytes. eNeuro 12(9) (2025) 40897540 |
Tsunematsu T, Sakata S, Sanagi T, Tanaka KF, Matsui K. Region-Specific and State-Dependent Astrocyte Ca2+ Dynamics during the Sleep-Wake Cycle in Mice. J Neurosci 41(25) 5440-5452(2021) 34006590 |
Chen J, He W, Hu X, Shen Y, Cao J, Wei Z, Luan Y, He L, Jiang F, Tao Y. A role for ErbB signaling in the induction of reactive astrogliosis. Cell Discov 3 17044(2017) 29238610 |