Strain Data Sheet

RBRC05447

Strain Information

Image
BRC No.RBRC05447
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(ACTB-tTS)1Mllo
Former Common nameActin-tTS Tg, B6-ACTB-tTS Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development田中先生が開発者Dr. Moises Malloより2008年に生理研へ導入。系統の由来 : 不明
Strain description全身の細胞でtTSを発現する。tetOをノックインしたホモ接合体との組み合わせで遺伝子ノックダウンが可能になる。
Colony maintenance入手後129S6へ10代バッククロスしてある。
ReferencesGenomics, 81, 356-360 (2003). 12676559
Nat. Commun., 12(1) 5146 (2021). 34446732
Biol. Psychiatry, 67, 770-773 (2010). 20163789

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTSE. coli Tetracycline repressor (tetR), human ZFP KRAB domain cDNAUNtTStransgene insertion 1, Moises Mallohuman beta-actin promoter, E. coli Tetracycline repressor (tetR), human Zfp KRAB domain

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signalUN

Ordering Information

供与核酸human beta actin promoter, tTS (E. coli Tetracycline repressor (tetR), human ZFP KRAB domain cDNA), chicken globin genomic DNA, SV40 polyA signal
Research applicationTet system
提供条件条件を付加する。
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEVELOPER (Dr. Moises Mallo, (Max-Plank Institute of Immunobiology) is requested.　Ｇｅｎｏｍｉｃｓ，　81（4）：356-360 2003. The RECIPIENT must　contact　the DEVELOPER in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 　　For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEVELOPER and must obtain a prior written consent from the DEVELOPER.
Depositor田中　謙二（慶應義塾大学）
Strain Status凍結精子のアイコン凍結精子
Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data