Strain Data Sheet

RBRC05424

Strain Information

Image
BRC No.RBRC05424
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Drd1<tm2(IL2RA/mVenus)Koba
Former Common nameB6-Drd1/IL2RAmVenus KI
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kazuto Kobayashi, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine in 2010. A human IL2RA and mVenus fusion gene was transferred into 129 derived ES cells. The mutant mice were crossed to C57BL/6J for several times.
Strain descriptionThe human IL2RA and mVenus fusion gene was inserted into the start codon of Drd1 gene.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Selective neural pathway targeting reveals key roles of thalamostriatal projection in the control of visual discrimination.
Kato S, Kuramochi M, Kobayashi K, Fukabori R, Okada K, Uchigashima M, Watanabe M, Tsutsui Y, Kobayashi K
J. Neurosci., 31, 17169-17179 (2011). 22114284

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Drd1
MGI:99578		dopamine receptor D19Drd1<tm2(IL2RA/mVenus)Koba>
MGI:5427817		targeted mutation 2, Kazuto Kobayashi
IL2RA human Interleukin-2 receptor alpha-subunit9IL2RA
mVenus Yellow Fluorescent Protein (Aequorea victoria)9mVenus
SV40 polyA signal9

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal neuron morphology (MP:0002882)

  • abnormal operant conditioning behavior (MP:0002803)

  • behavior/neurological phenotype (MP:0005386)
    • Detailed phenotype data

    Ordering Information

    Donor DNAMouse Dopamine D1 receptor (Drd1), SV40 PolyA signal, Human Interleukin-2 receptor alpha-subunit, Jellyfish Variant of enhanced yellow fluorescent protein (mVenus)
    Research applicationFluorescent Proteins/lacZ System
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorKazuto Kobayashi (Fukushima Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)

    BRC mice in Publications

    No Data