Strain Data Sheet

RBRC05423

Strain Information

Image
BRC No.RBRC05423
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Opalin<tm1Tfr>
Former Common nameB6-Opalin KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Fumio Yoshikawa and Teiichi Furuichi, RIKEN Brain Science Institute in 2005. The mutant mice were backcrossed to C57BL/6J over 15 generations.
Strain descriptionOpalin, oligodendrocytic myelin paranodal and inner loop protein gene knockout mice. Exons 2-6 of Opalin gene was replaced with a Frt-PGK-Neo-Frt-loxP cassette. Homozygous mutant mice show no obvious abnormality.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJsmSlc]
ReferencesPLoS ONE, 11(11): e0166732 (2016). 27855200
J. Biol. Chem., 283, 20830-20840 (2008). 18490449

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Opalinoligodendrocytic myelin paranodal and inner loop protein19Opalin

Ordering Information

Donor DNABacteriophage P1 loxP, E. coli Neomycin resistance gene, S. cerevisiae FRT, Mouse PGK promoter, pA, Opalin / Tmem10
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS ONE, 11(11): e0166732 (2016). doi: 10.1371/journal.pone.0166732. J. Biol. Chem., 283, 20830-20840 (2008). doi: 10.1074/jbc.M801314200.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. J Biol Chem. 2008 283(30):20830-40. The use of the BIOLOGICAL RESOURCE is strictly limited to academic, non-profit research institutes for academic non-profit research purposes. The RECEIPINT must submit the Material Transfer Agreement form to the DEPOSITOR and the RIKEN BRC prior to obtaining the BIOLOGICAL RESOURCE. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR while two years from deposit. The RECIPENT must not transfer the BIOLOGICAL RESOURCE to any third party. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorTeiichi furuichi (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data