Strain Data Sheet
RBRC05156
Strain Information | |
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| Image | |
| BRC No. | RBRC05156 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129S6-Gt(ROSA)26Sor<tm1(CAG-ChR2/mCherry)Imayo> |
| Former Common name | R26-CAG-LF-ChR2(C128A)-mCherry |
| H-2 Haplotype | |
| ES Cell line | TC1 [129S6/SvEvTac] |
| Background strain | |
| Appearance | |
| Strain development | 開発者 / 機関 : 今吉 格 先生・影山 龍一郎先生(Itaru Imayoshi, Ryoichiro Kageyama) / 京都大学ウィルス研究所 (Institute for Virus Research, Kyoto University)開発年 : 2010年 |
| Strain description | ChR2 (C128A) -mCherryが、Cre及びFLPの両方の組み換え後に発現するコンストラクトを、Rosa26遺伝子座にノックインしてある。発現レベルを増強する為に、プロモーターはCAGプロモーターを用いており、またChR2 (C128A) -mCherryの終止コドンとSV40pA配列の間にWPRE配列を挿入してある。(ChR2 (C128A) -mCherry is expressed after both Cre- and FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, ChR2 (C128A) -mCherry is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region.) |
| Colony maintenance | 戻し交配系統名 (ブリーダー名) : C57BL/6J |
| References | Neurosci. Res., 75(1):53-58 (2013). 22465523 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-COP4*C128A/mCherry)Imayo>targeted mutation 1, Itaru Imayoshi Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter ChR2channelrhodopsin-2 (Chlamydomonas reinhardtii)6ChR2CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHgrowth hormone polyadenylation signal6GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mCherryRed Fluorescent Protein (Discosoma sp.)6mCherry Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter puropuromycin acyltransferase (Streptomyces alboniger)6puro Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HSV thymidine kinase polyA signal6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 early polyA signal6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 late polyA signal6 |
Ordering Information | |
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| 供与核酸 | S. alboniger puromycin resistance gene, growth hormone polyadenylation signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, Bacteriophage P1 loxP sites, Yeast FRT (flippase recombination target) sites, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), Chlamydomonas Channelrhodopsin-2 mutant C128A cDNA, Discosoma sp. mCherry cDNA, mouse Gt(ROSA)26Sor genomic region |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| 提供条件 | 条件を付加する。 研究成果の公表にあたって寄託者の指定する文献を引用する。Neurosci. Res., 75(1):53-58 (2013). 研究成果の公表にあたって謝辞の表明を必要とする。 学術機関の学術研究に限る。分与希望者は事前に今吉格（E-mail: iimayosh@virus.kyoto-u.ac.jp）に連絡をとり、分与に関して許可を得ること。（提供承諾書（書式6）の提出は不要）。指定のMaterial Transfer Agreement（MTA）の締結が必要。 |
| Depositor | 今吉 格（国立大学法人京都大学） |
| Strain Status |  凍結胚 凍結精子 |
| Strain Availability | 凍結胚より作出したマウスを2~4ヶ月以内に提供可能 凍結精子を1ヶ月以内に提供可能 凍結胚を1ヶ月以内に提供可能 |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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