Strain Data Sheet

RBRC05147

Strain Information

Image
BRC No.RBRC05147
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-mTFP1)Imayo>
Former Common nameR26-CAG-LoxP-mTFP1
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strain
Appearance
Strain development開発者 / 機関 : 今吉 格 先生・影山 龍一郎先生(Itaru Imayoshi, Ryoichiro Kageyama) / 京都大学ウィルス研究所 (Institute for Virus Research, Kyoto University)開発年 : 2010年
Strain description蛍光たんぱく質mTFP1 (N末にHAタグを付加) が、Cre及びFLPの両方の組み換え後に発現するコンストラクトを、Rosa26遺伝子座にノックインしてある。発現レベルを増強する為に、プロモーターはCAGプロモーターを用いており、またmTFP1の終止コドンとSV40pA配列の間にWPRE配列を挿入してある。キメラマウスをpCAG-FLPeマウスと交配し、FRT-Stop-FRTカセットを除去した。(HAtag-attached mTFP1 fluorescent protein is expressed after Cre-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, HA-mTFP1 is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region.)
Colony maintenance戻し交配系統名 (ブリーダー名) : C57BL/6J
ReferencesNeurosci. Res., 73, 85-91 (2012). 22343123

Health Report

Examination Date / Room / Rack2024/02/19Room:4-ARack:J
2023/11/20Room:4-ARack:J
2023/08/21Room:4-ARack:J
2023/05/22Room:4-ARack:J
2023/02/20Room:4-ARack:J
2022/11/21Room:4-ARack:J
2022/08/22Room:4-ARack:J
2022/05/23Room:4-ARack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1(CAG-mTFP1)Imayo>targeted mutation 1.1, Itaru Imayoshi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHgrowth hormone polyadenylation signal6GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HAhuman influenza virus HA (hemagglutinin) tag sequence6HA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SASA (splice acceptor sequence)6SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mTFP1monomeric Teal Fluorescent Protein 1 (Clavularia sp.)6mTFP1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin acyltransferase (Streptomyces alboniger)6puro

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tkHSV thymidine kinase polyA signal6tk

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
STOP cassette (SV40pA-TKpA-SV40pA)6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 late polyA signal6

Ordering Information

供与核酸SA (splice acceptor sequence), S. alboniger puromycin resistance gene, growth hormone polyadenylation signal, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV-IE enhancer), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, Bacteriophage P1 loxP sites, Yeast FRT (flippase recombination target) site, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), HA (hemagglutinin) tag sequence, Clavularia sp. mTFP1, STOP cassette
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたって寄託者の指定する文献を引用する。Neurosci. Res., 73, 85-91 (2012).
学術機関の学術研究に限る。分与希望者は事前に今吉格（E-mail: iimayosh@virus.kyoto-u.ac.jp）に連絡をとり、分与に関して許可を得ること。（提供承諾書（書式6）の提出は不要）。
Depositor今吉　格（国立大学法人京都大学）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
生体マウスを1~3ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Itaru Imayoshi HP
Genotyping protocol -PCR-

BRC mice in Publications

Kase Y, Otsu K, Shimazaki T, Okano H.
Involvement of p38 in Age-Related Decline in Adult Neurogenesis via Modulation of Wnt Signaling.
Stem Cell Reports 12(6) 1313-1328(2019) 31080114