Strain Data Sheet

RBRC05118

Strain Information

Image
BRC No.RBRC05118
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6CB-Tnf<tm1Sek>
Former Common nameB6-Tnf KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development1997年 家畜衛生試験場 関川賢二先生らにより開発。TT2 ES細胞由来。東大医科研岩倉研でC57BL/6Jに12世代戻し交配 (兄妹交配3代) (BRC寄託時)。(C57BL/6 × CBA) F1 (TT2)
Strain descriptionTNF alpha遺伝子欠損マウス。細菌等に対して易感染性。
Colony maintenanceC57BL/6JJmsSlc
References
Failure of germinal center formation and impairment of response to endotoxin in tumor necrosis factor alpha-deficient mice.
Taniguchi T, Takata M, Ikeda A, Momotani E, Sekikawa K
Lab. Invest., 77, 647-658 (1997). 9426403

Health Report

Examination Date / Room / Rack2024/08/26Room:4-BRack:J囮検査
2024/05/27Room:4-BRack:J囮検査
2024/02/26Room:4-BRack:J囮検査
2023/11/27Room:4-BRack:J囮検査
2023/08/28Room:4-BRack:J囮検査
2023/05/29Room:4-BRack:J囮検査

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tnf
MGI:104798		tumor necrosis factor17Tnf<tm1Sek>
MGI:2653694		targeted mutation 1, Kenji Sekikawa
  • inherited susceptibility to asthma(MedGEN)

  • malaria, susceptibility to(MedGEN)

  • migraine with or without aura, susceptibility to, 1(MedGEN)
    • neo neomycin resistance gene (E. coli)17 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal Peyer's patch germinal center morphology(MP:0002391)

  • abnormal cytokine secretion(MP:0003009)

  • abnormal female reproductive system physiology(MP:0003699)

  • abnormal liver physiology(MP:0000609)

  • abnormal macrophage physiology(MP:0002451)
    • more 18 phenotypes
  • abnormal mesenteric lymph node morphology(MP:0005232)

  • abnormal serotonin level(MP:0005322)

  • abnormal vagina weight(MP:0008978)

  • absent lymph node germinal center(MP:0008523)

  • absent spleen germinal center(MP:0008474)

  • decreased circulating interferon-gamma level(MP:0008578)

  • decreased circulating interleukin-1 alpha level(MP:0008639)

  • decreased circulating interleukin-1 beta level(MP:0008642)

  • decreased circulating interleukin-6 level(MP:0008597)

  • decreased coping response(MP:0003064)

  • decreased locomotor activity(MP:0001402)

  • decreased susceptibility to endotoxin shock(MP:0008734)

  • decreased vertical activity(MP:0002757)

  • immune system phenotype(MP:0005387)

  • increased anxiety-related response(MP:0001363)

  • increased grooming behavior(MP:0001441)

  • increased serotonin level(MP:0010069)

  • prolonged estrus(MP:0009017)
    • Detailed phenotype data

    Ordering Information

    供与核酸mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Tnf genomic DNA
    Research applicationImmunology and Inflammation Research
    covid19_onset
    提供条件研究成果の公表にあたって寄託者の指定する文献を引用する。Lab. Invest., 77, 647-658 (1997). 研究成果の公表にあたって謝辞の表明を必要とする。
    Depositor岩倉　洋一郎（国立大学法人東京大学）
    Strain Status凍結胚のアイコン凍結胚
    凍結精子のアイコン凍結精子
    Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    凍結胚を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data