Strain Data Sheet

RBRC04940

Strain Information

Image
BRC No.RBRC04940
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(Ins2-Tnf)
Former Common nameB6-Ins2-Tnf Tg (TNF-Tg)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentB6D2F2受精卵へのインジェクションにより作製、C57BL/6Nマウスに戻し交配(N11)後、兄妹交配(ホモ型)にて維持されていたもの。
Strain descriptionラットinsulinプロモーター制御下で、マウスTNF alpha を発現するトランスジェニックマウス。膵臓 beta 細胞にTNF alpha を発現し、3週齢からラ氏島にリンパ球浸潤を認め、3ヶ月齢以降より強いinsulitisを1年以上示す。糖尿病の発症は認められない。
Colony maintenance通常の維持繁殖には特に問題ない。
References
Expression of a tumor necrosis factor alpha transgene in murine pancreatic beta cells results in severe and permanent insulitis without evolution towards diabetes.
Higuchi Y, Herrera P, Muniesa P, Huarte J, Belin D, Ohashi P, Aichele P, Orci L, Vassalli J D, Vassalli P
J. Exp. Med., 176, 1719-1731 (1992). 1460428

Induction of diabetes is influenced by the infectious virus and local expression of MHC class I and tumor necrosis factor-alpha.
Ohashi P S, Oehen S, Aichele P, Pircher H, Odermatt B, Herrera P, Higuchi Y, Buerki K, Hengartner H, Zinkernagel R M
J. Immunol., 150, 5185-5194 (1993). 8496610

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tnf
MGI:104798
tumor necrosis factorUNTnf rat Insulin prommoter 2, SV40 polyA signal
  • inherited susceptibility to asthma(MedGEN)

  • malaria, susceptibility to(MedGEN)

  • migraine with or without aura, susceptibility to, 1(MedGEN)
  • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal pancreatic islet morphology(MP:0005215)

  • homeostasis/metabolism phenotype(MP:0005376)

  • immune system phenotype(MP:0005387)

  • insulitis(MP:0004031)
  • Detailed phenotype data

    Ordering Information

    供与核酸Mouse TNF-alpha genomic DNA, Rat Insulin prommoter 2, SV40 polyA signal
    Research applicationDiabetes and Obesity Research
    covid19_onset
    covid19_progression
    提供条件条件を付加しない。
    Depositor樋口 安典(国立大学法人大分大学)
    Strain Status凍結精子のアイコン凍結精子
    Strain Availability凍結精子より作出したマウスを2~4ヶ月以内に提供可能
    凍結精子を1ヶ月以内に提供可能
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data