Strain Information | |
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Image | |
BRC No. | RBRC04895 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6(Cg)-Timd4<tm1Osa> |
Former Common name | B6-Tim4 KI/Venus |
H-2 Haplotype | |
ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
Background strain | |
Appearance | |
Strain development | Developed by Masanori Miyanishi and Shigekazu Nagata, Graduate School of Medicine, Kyoto University in 2007. |
Strain description | Original reference: Miyanishi M, et al., Synergistic effect of Tim4 and MFG-E8 null mutations on the development of autoimmunity. Int Immunol. 2012 Sep;24(9):551-9 |
Colony maintenance | Heterozygote x Wild-type [C57BL/6J(Crlj)] |
References | Identification of Tim4 as a phosphatidylserine receptor. Miyanishi M, Tada K, Koike M, Uchiyama Y, Kitamura T, Nagata S Nature, 450, 435-439 (2007). 17960135Synergistic effect of Tim4 and MFG-E8 null mutations on the development of autoimmunity. Miyanishi M, Segawa K, Nagata S Int. Immunol. 24(9):551-9 (2012). 22723547 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Timd4 | T cell immunoglobulin and mucin domain containing 4 | 11 | Timd4 | targeted mutation 1, Shigekazu Nagata | |||
Venus | Yellow Fluorescent Protein (Aequorea victoria) | 11 | Venus | ||||
loxP | phage P1 loxP | 11 | loxP | ||||
loxP | phage P1 loxP | 11 | loxP | ||||
neo | neomycin resistance gene (E. coli) | 11 | mouse phosphoglycerate kinase promoter (PGK promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | Bacteriophage P1 loxP sites, Mouse Phosphoglycerate kinase-1 promoter, E.coli Neomycin resistance gene, Aequorea victoria Venus gene (GFP derivagtive), Simian virus 40 polyA, mouse Timd4 genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 450, 435-439 (2007). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results within two (2) years after deposit of the BIOLOGICAL RESOURCE, the RECIPIENT must include the DEPOSITOR as co-authors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
Depositor | Shigekazu Nagata (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Chow A, Schad S, Green MD, Hellmann MD, Allaj V, Ceglia N, Zago G, Shah NS, Sharma SK, Mattar M, Chan J, Rizvi H, Zhong H, Liu C, Bykov Y, Zamarin D, Shi H, Budhu S, Wohlhieter C, Uddin F, Gupta A, Khodos I, Waninger JJ, Qin A, Markowitz GJ, Mittal V, Balachandran V, Durham JN, Le DT, Zou W, Shah SP, McPherson A, Panageas K, Lewis JS, Perry JSA, de Stanchina E, Sen T, Poirier JT, Wolchok JD, Rudin CM, Merghoub T. Tim-4+ cavity-resident macrophages impair anti-tumor CD8+ T cell immunity. Cancer Cell 39(7) 973-988.e9(2021) 34115989 |
Lee SA, Kim D, Min C, Moon B, Lee J, Moon H, Yang S, Lee CS, Lee G, Park D. Phagocyte Chemoattraction Is Induced through the Mcp-1-Ccr2 Axis during Efferocytosis. Cells 10(11) (2021) 34831339 |