Strain Data Sheet

RBRC04874

Strain Information

Image
BRC No.RBRC04874
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Gt(ROSA)26Sor<tm1(CAG-EGFP/tDsRed)Utr>/Rbrc
Former Common name
H-2 Haplotype
ES Cell lineB6N-22<UTR> [C57BL/6NCrlCrlj]
Background strainC57BL/6NCrlCrlj
Appearance
Strain development筑波大学生命科学動物資源センターおよび理化学研究所バイオリソースセンター（2011）。B6N由来ES細胞（B6N-22<Utr>）を用いて作出（#9クローン由来）。
Strain descriptionR26GRRノックインマウス。Cre遺伝子組換えにより緑色蛍光が赤色蛍光に変換するコンストラクトがRosa26遺伝子座に挿入されている。
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6NCrl]
ReferencesExp. Anim., 71(3), 385-390 (2022). 35444103
BMC Res. Notes 16(1) 109 (2023). 37337280
Exp. Anim., 62, 295-304 (2013). 24172193
BMC Res. Notes 16(1) 54 (2023). 37069662

Health Report

Examination Date / Room / Rack2024/01/09Room:3-BRack:F
2023/10/10Room:3-BRack:F
2023/07/10Room:3-BRack:F
2023/04/10Room:3-BRack:F
2023/01/10Room:3-BRack:F
2022/10/11Room:3-BRack:F
2022/07/11Room:3-BRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA26)Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA26)Sor<tm1(CAG-EGFP/DsRed)Utr>targeted mutation 1 (CAG-EGFP/DsRed), University of Tsukuba Laboratory Animal Resource Center

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)6FLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tDsRedRed Fluorescent Protein (Discosoma sp.)6tDsRed

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA6

Ordering Information

供与核酸Rosa gene (ubiquitous) (mouse), CMV IE enhancer (cytomegalovirus), beta-actin promoter (chicken), beta-globin genomic DNA (rabbit), EGFP (jellyfish), DsRed (red flourescent protein from Discosoma sp.) cDNA, PGK promoter (mouse), neomycin resistance gene (E. coli), polyA additional signal (mouse), FRT sites (yeast), loxP sites (P1 phage), phage T7 gb2 promoter(synthetic), polyA (SV40), FLAG tag (synthetic)
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
研究成果の公表にあたっては、八神健一（筑波大学生命科学動物資源センター）と理研バイオリソースセンターに対して謝辞の表明を必要とする。研究成果の公表にあたって寄託者の指定する文献を引用する。Exp Anim 62, 295-304 (2013). 学術機関の学術研究に限る。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
DepositorRIKEN BRC（独立行政法人理化学研究所）
Strain Status生体のアイコン生体
凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
生体マウスを3~6ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Mar 2014, Mouse of the Month Oct 2018

BRC mice in Publications

Hashimoto M, Sasaki H.
Epiblast Formation by TEAD-YAP-Dependent Expression of Pluripotency Factors and Competitive Elimination of Unspecified Cells.
Dev Cell 50(2) 139-154.e5(2019) 31204175

Shibata M, Banno R, Sugiyama M, Tominaga T, Onoue T, Tsunekawa T, Azuma Y, Hagiwara D, Lu W, Ito Y, Goto M, Suga H, Sugimura Y, Oiso Y, Arima H.
AgRP Neuron-Specific Deletion of Glucocorticoid Receptor Leads to Increased Energy Expenditure and Decreased Body Weight in Female Mice on a High-Fat Diet.
Endocrinology 157(4) 1457-66(2016) 26889940

Matsumoto L, Hirota Y, Saito-Fujita T, Takeda N, Tanaka T, Hiraoka T, Akaeda S, Fujita H, Shimizu-Hirota R, Igaue S, Matsuo M, Haraguchi H, Saito-Kanatani M, Fujii T, Osuga Y.
HIF2α in the uterine stroma permits embryo invasion and luminal epithelium detachment.
J Clin Invest 128(7) 3186-3197(2018) 29911998

Hiraoka T, Hirota Y, Saito-Fujita T, Matsuo M, Egashira M, Matsumoto L, Haraguchi H, Dey SK, Furukawa KS, Fujii T, Osuga Y.
STAT3 accelerates uterine epithelial regeneration in a mouse model of decellularized uterine matrix transplantation.
JCI Insight 1(8) (2016) 27358915

Mizoguchi A, Banno R, Sun R, Yaginuma H, Taki K, Kobayashi T, Sugiyama M, Tsunekawa T, Onoue T, Takagi H, Hagiwara D, Ito Y, Iwama S, Suga H, Nagai T, Yamada K, Arima H.
Glucocorticoid receptor signaling in ventral tegmental area neurons increases the rewarding value of a high-fat diet in mice.
Sci Rep 11(1) 12873(2021) 34145364

Tsunekawa T, Banno R, Yaginuma H, Taki K, Mizoguchi A, Sugiyama M, Onoue T, Takagi H, Hagiwara D, Ito Y, Iwama S, Goto M, Suga H, Bettler B, Arima H.
GABAB Receptor Signaling in the Mesolimbic System Suppresses Binge-like Consumption of a High-Fat Diet.
iScience 20 337-347(2019) 31610370