Strain Data Sheet

RBRC04850

Strain Information

Image
BRC No.RBRC04850
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6C3(129)-Tg(NDE-hsp-Pkd2*R6GdC/Venus)101Hmd
Former Common nameNDE-Pkd2ml(delta)C-Venus(101)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Satoko Yoshiba and Dr. Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010 The transgene was injected into the pronuclei of (C57BL/6 x C3H) F1 fertelized eggs. C57BL/6, C3H and 129 mized background.
Strain descriptionThese transgenic mice express the Arg6Gly variant Pkd2 and venus on both nude under the control of the node specific enhancer.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References
Cilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2.
Yoshiba S, Shiratori H, Kuo I Y, Kawasumi A, Shinohara K, Nonaka S, Asai Y, Sasaki G, Belo J A, Sasaki H, Nakai J, Dworniczak B, Ehrlich B E, Pennekamp P, Hamada H
Science, 338, 226-231 (2012). 22983710

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Pkd2
MGI:1099818		polycystic kidney disease 2UNPkd2 mouse node-specific enhancer, hsp promoter
  • autosomal dominant polycystic kidney disease(DisGeNET)

  • polycystic kidney disease 2(DisGeNET, MedGEN)
    • Venus Yellow Fluorescent Protein (Aequorea victoria)UNVenus

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAマウス由来Nodalのノードエンハンサー, 変異型Pkd2cDNA, hspプロモーター, 改変型クラゲ由来EGFP
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 338, 226-231 (2012).In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data