Strain Data Sheet

RBRC04801

Strain Information

Image
BRC No.RBRC04801
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Kcp<tm1Ysas>/Rbrc
Former Common nameKcp(tau-lacZ) (Acc.No.CDB0401K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Dr. Yoshiki Sasai, Center for Developmental Biology, RIKEN.
Strain descriptionThis transgene contains tau-lacZ with a floxed neo in the first exon of Kcp to replace with lacZ at the first ATG. Kcp is a Kielin-related molecule that contains multiple Chd-type cysteine-rich repeats, a vWD domain and a TIL domain, and physically binds to Bmp proteins. Homozygous mutant mice are viable and fertile, and exhibit no gross structural defects.
Colony maintenanceHeterozygote X Wild-type [C56BL/6] Homozygous mutant mice are viable and fertile.
ReferencesDevelopment, 133, 4463-4473 (2006). 17035289

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kcpkielin/chordin-like protein6Kcp<tm1Ysas>targeted mutation 1, Yoshiki Sasai

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly ASV40 polyA6poly A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
taubovine tau protein cDNA6tau

Ordering Information

供与核酸bovine tau cDNA, E. coli lacZ, SV40 polyA, phage P1 loxP sites, mouse PGK promoter, E. coli neo, mouse Kcp genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
提供条件条件を付加する。
(1) 分与希望者は事前に池谷　真 (mikeya@cira.kyoto-u.ac.jp) に連絡をとり、分与に関して許可を得ること。(2) 当該マウスを用いる論文発表に際しては Accession No. CDB0401K、その WEB サイト（https://large.riken.jp/distribution/mutant-list.html）とともに当該マウスを記載すること。(3) 当該マウスを用いる論文発表に際しては、その由来として第一報論文（Development, 133, 4463-4473 (2006).）を引用すること。(4)発表論文を寄託者 (mutant.bdr@riken.jp)に連絡すること。
DepositorRIKEN CDB（独立行政法人理化学研究所）
Strain Status凍結胚のアイコン凍結胚
凍結精子のアイコン凍結精子
Strain Availability凍結胚より作出したマウスを2~4ヶ月以内に提供可能
凍結精子を1ヶ月以内に提供可能
凍結胚を1ヶ月以内に提供可能
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data